Peptide Mapping

Dr. Anne Zeck
Group Leader Bioanalytics

+49 7121 51530-811

E-Mail

anzeigen

Peptide mapping and determination of post-translational modifications of recombinant proteins (antibodies)

Peptide mapping is used to verify the primary amino acid sequence of a protein according to its DNA sequence. In addition, post-translational modifications (PTMs) such as the glycosylation pattern are examined and residual host cell proteins are identified. Different production batches can also be compared in detail.

Our services

• Verification of primary amino acid sequence and labelling of peptides in the chromatogram
• Identification, localization and relative quantification of PTMs and degradations (e.g. N- and O-glycosylation, glycation, oxidation, deamidation)
• Differential comparison of different production batches
• Identification of host cell proteins

Methods

• Tryptic PeptideMap with HPLCL-MS/MS
• Relative quantification of degradation hot-spots