Antibody Internalization

Dr. Frank Weise
Group Leader Molecular- und Neurobiology

+49 7121 51530-56

E-Mail

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Dr. Martin Kriebel
Molecular and Neurobiology

+49 7121 51530-810

E-Mail

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Monoclonal antibodies allow innovative, targeted therapy approaches in oncology

Monoclonal antibodies are used as innovative therapeutic tools in oncology and other indications. In preclinical development, in addition to a demonstration of efficacy, further parameters such as the dynamics of epitope-specific cellular uptake or the intracellular processing of therapeutic immunoglobulins are to be determined.

Our services

• Provision of cellular assay systems for the analysis of binding and downstream internalization of monoclonal antibodies
• Time-resolved documentation of the internalization process
• Determination of internalization parameters by quantitative image analysis including 3D/4D reconstructions of the internalization process
• Elucidation of intracellular processing of internalized antibodies

Methods

• Confocal fluorescence and live cell microscopy

• Quantitative analysis of microscopic image data

• 3D and 4D reconstructions and animations

Instruments

• Microscopy: Carl Zeiss CLSM + Cell Observer SD, Molecular Devices ImageXpress Micro confocal

• Software: Oxford Instruments IMARIS 10, Molecular Devices MetaXpress 6.7 

Methods

• Confocal fluorescence and live cell microscopy
• Quantitative analysis of microscopic image data
• 3D and 4D reconstructions and animations

Devices

• Mikroskopie: Carl Zeiss CLSM + Cell Observer SD, Molecular Devices ImageXpress Micro konfokal
• Analyse-Software: Oxford Instruments IMARIS 10, Molecular Devices MetaXpress 6.7