Marker Independent Microscopy

Dr. Julia Marzi
Group Leader Biophotonics & Spectroscopy

Non-destructive imaging techniques for biomaterials and biological samples

Using endogenous fluorophores and molecule-specific spectral signatures - comparable to a characteristic fingerprint - images and data can be collected without affecting the sample state and also enables time-resolved, dynamic measurements.


Our services

Establishing and performing non-destructive and marker-independent microscopic procedures on synthetic and biological samples, especially for biomedical issues, such as:

  • In vitro and in vivo degradation of biodegradable (implant) materials
  • Real-time monitoring of pharmacological effects in in vitro cell culture models
  • Investigation of morphological and molecular changes, e.g. in collagens



  • Raman Spectroscopy & Microscopy, SERS and CARS Spectroscopy
  • Multiphoton-Microscopy (TPEF, SHG, THG)
  • Fluorescence- und Phosphorescence-Liftime Microscopy (FLIM, PLIM)
  • Confocal Flourescence Microscopy
  • 3D Image Reconstruction
  • Multivariate data analysis and machine learning
  • Spectral biomolecule databases



  • InVia Raman Spectrometer
  • Carl Zeiss LSM880