Biomarker and Assay Development

Unlock the secrets of biological changes with our accurate and reliable antibody-based detection systems

Biological or pathological processes lead to changes in the composition of the proteome. Accurate analysis of such changes is performed by biomarker analysis using antibody-based detection systems. We offer high-quality customized analytical solutions ranging from conventional ELISAs to highly sensitive immunoassays for exactly this purpose.

 

Our services

Development of sandwich immunoassays on multiple platforms:

• Preparation of reagents for immunoassays

• Optimization of assay reagents

• Fine-tuning of assay conditions

• Development of prototype assays or fully optimized assays

• From conventional to ultrasensitive immunoassays

• Increase efficiency and throughput rates through automation

• Reliable and consistent results through standardized reagent preparation

 

Available technologies

• Singleplex and multiplex bead-based immunoassays (Luminex® platform).

• Enzyme-linked immunosorbent assay (ELISA)

• Ultrasensitive bead-based immunoassays (SIMOA® HD-X platform from Quanterix^TM)

• Ultrasensitive planar immunoassays (SIMOA® SP-X platform from Quanterix^TM)

• High- and ultrasensitive immunoassays on the SIMOA® platform under GCP regulations.

 

Services

• GCP-compliant analysis of clinical trial samples with highly sensitive immunoassays (SIMOA HD-X, SIMOA SP-X platforms).
• Analysis of exploratory, preclinical and clinical study samples with commercially available assay kits (e.g. Quanterix/SIMOA®, Luminex®, ELISA)
• Biomarker identification
• Evaluation of assay technologies
• Consulting on immunoassay technologies and protein-protein interaction analysis
• Automation of immunoassays and reagent preparation
• Customized assay validations according to EMA/FDA/ICH or CLSI guidelines
• Electrophoresis (SDS-PAGE, Western blot) for protein analysis

 

Methods

• Multiplexe Immunoassays
• Singleplexe Immunoassays
• Hoch-/Ultrasensitive Immunoassays
• ELISAs

 

Instruments

• Multiple Luminex FLEXMAP 3D® and Luminex® xMAP® INTELLIFLEX instruments
• Multiple Quanterix® HD-X instruments
• Quanterix® SP-X Instrument
• ELISA Reader
• Kingfisher Flex (Thermo Scientific) automated bead-handling systems

Services

• GCP-compliant analysis of clinical trial samples with highly sensitive immunoassays (SIMOA HD-X, SIMOA SP-X platforms)
• Analysis of exploratory, preclinical and clinical study samples with commercially available assay kits (e.g. Quanterix/SIMOA®, Luminex®, ELISA)
• Biomarker identification
• Evaluation of assay technologies
• Consulting on immunoassay technologies and protein-protein interaction analysis
• Automation of immunoassays and reagent production
• Customized assay validations according to EMA/FDA/ICH or CLSI guidelines
• Electrophoresis (SDS-PAGE, Western blot) for protein analysis