Testing the identity, integrity and labeling level of recombinant proteins

Analysis of intact proteins by mass spectrometry

We offer a rapid MS method to verify the identity and integrity of recombinantly produced proteins

 

Our services

  • Verification of identity based on the exact protein mass
  • Verification of integrity
    • Protein processing by enyzmes
    • Mismatches of bispecific antibodies
    • Degradation in the context of stability studies
    • Determination of degree of labelling of protein conjugates
  • Determination and relative quantification of PTMs with delta mass >15 Da

 

Methods

  • Deglycosylation, denaturation, reduction as required
  • LC-TOF-MS analysis
  • Charge deconvolution and data analysis

 

Publications

  • Oroszlán G, Kortvely E, Szakács D, Kocsis A, Dammeier S, Zeck A, Ueffing M, Závodszky P, Pál G, Gál P, Dobó J. MASP-1 and MASP-2 Do Not Activate Pro-Factor D in Resting Human Blood, whereas MASP-3 Is a Potential Activator: Kinetic Analysis Involving Specific MASP-1 and MASP-2 Inhibitors. J Immunol. 2016 Jan 15;196(2):857-65. doi: 10.4049/jimmunol.1501717. Epub 2015 Dec 16. PMID: 26673137.