Dr. Anne Zeck
Group Leader Bioanalytics
Analysis of intact proteins by mass spectrometry
We offer a rapid MS method to verify the identity and integrity of recombinantly produced proteins
Our services
- Verification of identity based on the exact protein mass
- Verification of integrity
- Protein processing by enyzmes
- Mismatches of bispecific antibodies
- Degradation in the context of stability studies
- Determination of degree of labelling of protein conjugates
- Determination and relative quantification of PTMs with delta mass >15 Da
Methods
- Deglycosylation, denaturation, reduction as required
- LC-TOF-MS analysis
- Charge deconvolution and data analysis
Publications
- Oroszlán G, Kortvely E, Szakács D, Kocsis A, Dammeier S, Zeck A, Ueffing M, Závodszky P, Pál G, Gál P, Dobó J. MASP-1 and MASP-2 Do Not Activate Pro-Factor D in Resting Human Blood, whereas MASP-3 Is a Potential Activator: Kinetic Analysis Involving Specific MASP-1 and MASP-2 Inhibitors. J Immunol. 2016 Jan 15;196(2):857-65. doi: 10.4049/jimmunol.1501717. Epub 2015 Dec 16. PMID: 26673137.