Biological or pathological processes lead to changes in the composition of the proteome. The accurate analysis of such changes of individual proteins/biomarkers can be performed using antibody-based detection systems. The Multiplex Immunoassays Group at the NMI uses and develops multiplex immunoassays for this purpose. Multiplexing enables the analysis of multiple biomarkers simultaneously. Based on their information-density, multiplex assays can be applied in early/differential diagnosis, disease staging or epidemiological studies, as well as in vaccine or therapeutic development. The Multiplex Immunoassays Group contributes its technical and scientific know-how to industrial research cooperations and joint projects with partners from industry and science.
Services
Our services are tailor-made to meet the requirements of our customers.
Multiplex Immunoassays
- Development of Multiplex Immunoassays on Luminex Platform and/or all required assay reagents
- Assay Validation (fit-for-purpose, according to EMA/FDA or CLSI guidelines)
- Sample analysis of exploratory, preclinical and clinical study samples
- Sample analysis with commercially available assay kits (e.g. Luminex®)
- Sample analysis with in-house developed antibody assays for SARS-CoV-2, Borrelia, Hepatitis and RSV. Further assays in development include Influenza and Measles.
- biomarker identification
- Assay and technology evaluation
- Advice on immunoassay technologies
- Epitope Mapping
- Peptide Arrays
Sandwich immunoassays
- Development of ELISAs and/or all required assay reagents
- Assay Validation
- Sample analysis including with commercially available kits (e.g. ELISA)
- biomarker identification
- Assay and technology evaluation
- Advice on immunoassay technologies
General biochemical methods
- Protein-protein interaction analysis
- Enzyme substrate assays
- Antibody generation (polyclonal, monoclonal)
- Purification of antibodies and proteins
- Antibody characterization: thermal stability and aggregation (nanoDSF, Prometheus), epitope mapping, affinity (Biacore®)
Techniques, methods, equipment
Depending on the task, state-of-the-art analytical equipment is used to achieve optimum results in terms of efficiency, costs, throughput and quality. A semi-automated laboratory set up is available for high-throughput bead coupling and sample measurement (upto 8000 per week).
Immunoassays
- several Luminex FLEXMAP 3D® instruments
- Luminex INTELLIFLEX instrument
- Beckman Coulter i7 pipetting robot
- Luminex MAGPIX®
- 2 Quanterix® HD-1 Instruments
- Quanterix® SRX Instrument
- ELISA Reader
Electrophoresis
- SDS-PAGE, Western Blot
Chromatography systems
- 3 ÄKTAxpress (GE Healthcare)
Antibody characterization
- Biacore® 3000
- Prometheus
- OctetRED
Network
Universities:
e.g. Tübingen, Ulm, Freiburg, Hannover Medical School, Hamburg
Clinics:
e.g. Universitätsklinikum Tübingen, University of Tübingen Centre for Neurology, University Hospital Freiburg, Charite Berlin, Medecine Interne et Maladies Infectieuses - Paris
Research institutes:
e.g. Helmholtz Centre for Infection Research, Swiss Tropical and Public Health Institute, Hertie Institute for Clinical Brain Research