Multiplex Immunoassays

Biological or pathological processes lead to changes in the composition of the proteome. The accurate analysis of such changes of individual proteins/biomarkers can be performed using antibody-based detection systems. The group uses miniaturized and parallel immunoassays for this purpose. The Multiplex Immunoassays working group contributes its technical and scientific know-how to industrial research cooperations and joint projects with partners from industry and science.


The services are tailor-made for the questions of the customers.

Sandwich immunoassays

  • Development of
    • multiplex immunoassays (Luminex® platform)
    • ELISAs
    • all required assay reagents
  • Assay Validation (fit-for-purpose, according to EMA/FDA or CLSI guidelines)
  • Sample analysis of exploratory, preclinical and clinical study samples
  • Sample analysis with commercially available assay kits (Luminex®, ELISA)
  • biomarker identification
  • Assay and technology evaluation
  • Advice on immunoassay technologies

Multiplex antigen arrays

  • Development of antigen arrays and multiplex serological immunoassays (Luminex)
  • Assay Validation
  • Sample analysis
  • Epitope Mapping
  • Peptide Arrays

General biochemical methods

  • Protein-protein interaction analysis
  • Enzyme substrate assays
  • Antibody generation (polyclonal, monoclonal)
  • Purification of antibodies and proteins
  • Antibody characterization: thermal stability and aggregation (nanoDSF, Prometheus), epitope mapping, affinity (Biacore®)

Techniques, methods, equipment

Depending on the task, state-of-the-art analytical equipment is used to achieve optimum results in terms of efficiency, costs, throughput and quality.


  • several Luminex FLEXMAP 3D® instruments
  • Luminex MAGPIX®
  • 2 Quanterix® HD-1 Instruments
  • Quanterix® SRX Instrument
  • ELISA Reader


  • SDS-PAGE, Western Blot

Chromatography systems

  • 3 ÄKTAxpress (GE Healthcare)

Antibody characterization

  • Biacore® 3000
  • Prometheus


e.g. Tübingen, TU Munich, Ulm, Freiburg, Royal Institute of Technology, Lund University, Harvard Medical School,

e.g. University of Tübingen Centre for Neurology, Charite Berlin, Medecine Interne et Maladies Infectieuses, Paris,

Research institutes:
Hertie Institute for Clinical Brain Research, Science for Life Laboratory, Stockholm; Pasteur Institute, Paris; Barcelona Cardiovascular Research Center