Mole­cu­lar Biology and Neuro­bio­logy

Neurodegenerative as well as psychiatric diseases are associated with decreased/increased synaptic connectivity in selected brain areas. We characterize proteins involved in the  (de-)stabilization of synapses. We are engaged both in industrial contract research (Target research, drug development) and in joint projects with industrial and academic partners.

Application of our techniques is documented in high ranking publications

Techniques, Methods, Equipment

Test systems- CNS diseases:

  • Rats in vivo
  • Organotypic slice cultures
  • Dissociated neurons

Molecular biology routines:

  • RNA Interference (siRNA, miRNA, shRNA)
  • Recombinant protein expression
  • Tissue-specific promoters for targeted expression
  • Fluorescent Proteins

Gene transfer methods:

  • Viral systems (lentiviral, adenoviral, retroviral)
  • Stereotactic injection of lentivirus in vivo
  • Non-viral systems (Lipofection, Nucleofection)


Available Analyses:

  • Immunostaining for neuronal differentiation, synaptic density
  • Fluorescence microscopy, laser scan microscopy
  • Imaging incl. 3D reconstruction of labelled neurons
  • Common assays: viability, apoptosis, qRT-PCR, ROS, Reporterassays, signalling, protein interaction
  • Assay development for HCS application

Services:

  • Target identification via siRNA libraries
  • Target validation via overexpression or RNA interference
  • Development of viral expression systems
  • Drug testing in neuronal systems

Network

Universities
Tübingen, Hannover, Berlin, Freiburg, Würzburg, Haifa, London, Dublin, Kopenhagen, Tartu, Milton Keynes, Genf, Paris

Research institutes