Testing the identity, integrity and labeling level of recombinant proteins

Dr. Anne Zeck
Group Leader Bioanalytics

+49 7121 51530-811

E-Mail

anzeigen

Analysis of intact proteins by mass spectrometry

We offer a rapid MS method to verify the identity and integrity of recombinantly produced proteins

Our services

• Verification of identity based on the exact protein mass
• Verification of integrity
  • Protein processing by enyzmes
  • Mismatches of bispecific antibodies
  • Degradation in the context of stability studies
  • Determination of degree of labelling of protein conjugates
• Determination and relative quantification of PTMs with delta mass >15 Da

Methods

• Deglycosylation, denaturation, reduction as required
• LC-TOF-MS analysis
• Charge deconvolution and data analysis

Publications

• Oroszlán G, Kortvely E, Szakács D, Kocsis A, Dammeier S, Zeck A, Ueffing M, Závodszky P, Pál G, Gál P, Dobó J. MASP-1 and MASP-2 Do Not Activate Pro-Factor D in Resting Human Blood, whereas MASP-3 Is a Potential Activator: Kinetic Analysis Involving Specific MASP-1 and MASP-2 Inhibitors. J Immunol. 2016 Jan 15;196(2):857-65. doi: 10.4049/jimmunol.1501717. Epub 2015 Dec 16. PMID: 26673137.