Bio­chemistry

Biological or pathological processes lead to changes in the composition of the proteome. The accurate analysis of such changes of individual proteins/biomarkers can be performed using antibody-based detection systems. The group uses miniaturized and parallel immunoassays for this purpose. The Biochemistry Working Group contributes its technical and scientific know-how to industrial research cooperations and joint projects with partners from industry and science.

Services

The services are tailor-made for the questions of the customers.

Sandwich immunoassays

  • Development of
    • multiplex immunoassays (Luminex® platform)
    • ultrasensitive immunoassays (SIMOA platform from Quanterix®)
    • ELISAs
    • all required assay reagents
  • Assay Validation (fit-for-purpose, according to EMA/FDA or CLSI guidelines
  • Sample analysis of exploratory, preclinical and clinical study samples
  • Sample analysis with commercially available assay kits (SIMOA, Luminex®, ELISA)
  • biomarker identification
  • Assay and technology evaluation
  • Advice on immunoassay technologies

Multiplex antigen arrays

  • Development of antigen arrays and multiplex serological immunoassays (Luminex)
  • Assay Validation
  • sample analysis

General biochemical methods

  • protein-protein interaction analysis
  • enzyme substrate assays
  • Antibody generation (polyclonal, monoclonal)
  • Purification of antibodies and proteins
  • Antibody characterization: thermal stability and aggregation (nanoDSF, Prometheus), epitope mapping, affinity (Biacore®)

Techniques, methods, equipment

Depending on the task, state-of-the-art analytical equipment is used to achieve optimum results in terms of efficiency, costs, throughput and quality.

Immunoassays

  • several Luminex FLEXMAP 3D® instruments
  • Luminex MAGPIX®
  • 2 Quanterix® HD-1 Instruments
  • Quanterix® SRX Instrument
  • ELISA Reader

Electrophoresis

  • SDS-PAGE, Western Blot

Chromatography systems

  • 3 ÄKTAxpress (GE Healthcare)

Antibody characterization

  • Biacore® 3000
  • Prometheus

Network

Universities:
e.g. Tübingen, TU Munich, Ulm, Freiburg, Royal Institute of Technology, Lund University, Harvard Medical School,

Clinics:
e.g. University of Tübingen Centre for Neurology, Charite Berlin, Medecine Interne et Maladies Infectieuses, Paris,

Research institutes:
Hertie Institute for Clinical Brain Research, Science for Life Laboratory, Stockholm; Pasteur Institute, Paris; Barcelona Cardiovascular Research Center

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