Drug Target Gene Expression

Drug target gene expression technologies - Knockdown, Knockout, Overexpression

Target proteins (drug targets) are either (inducibly) overexpressed or their expression is inhibited (knock-down, knock-out). In this way, model systems are established that can be used for target validation, functional analysis and drug screening.

Our services

To support your target validation activities we offer:

• Cloning of your gene of interest into a suitable expression vector
• selection of promoters of different strengths
• lentiviral transduction of a cell culture of your choice
• selection of stable recombinant cell lines (polyclonal or monoclonal) and validation by gene expression analysis (on the mRNA or protein level)
• Characterization with regard to target protein localization
• Functional characterization (e.g. cell proliferation, apoptosis, metabolism, other assays on request)

We offer freedom to operate due to licenses for lentiviral technology, CRISPR/Cas9 & RNA interference technologies.

Since 2001 we have

> 250 cDNA expression vectors,

> 850 stable recombinant cell lines for the expression of

> 200 target proteins, and generated

used > 100 parental lines or primary cell cultures.

Methods

• Gene expression analysis (qRT-PCR, Western blot)
• Immunofluorescence Microscopy
• Flow Cytometry
• ELISA, Homogenous Time-resolved Fluorescence (HTRF)

Instruments

• Flow Cytometry: BD FACSmelody, BD LSR Fortessa
• Microscopy: CLSM (Zeiss), ImageXpress Micro confocal (Molecular Devices)
• Western blot: Typhoon Trio imager, LI-COR Odyssey
• Real time PCR: StepOnePlus, 7500 Fast
• ELISA, HTRF: Tecan Spark, BMG Labtech PHERAstar