Ihr Ansprechpartner
Dr. Frank Weise

Tel: 07121 51530-56

Target expression systems

Syntenin overexpression


The Target Expression Systems group focuses on the functional analysis of proteins within the scope of target identification / target validation for the pharmaceutical industry. To this end, we apply a panel of gene transfer methods in order to overexpress or knock-down gene expression in eukaryotic cells.

Furthermore, these techniques are used to unravel the mode of action of new compounds.


Track record: Expertise in gene transfer and expression analysis (2001-2015):

>250 cDNA expression vectors generated

>1.200 shRNA vectors for >250 targets validated

Techniques, methods, equipment

RNA interference (RNAi)

  • siRNA
  • shRNA
  • miRNA

Gene transfer

  • plasmid-based
  • adeno-, retro-, lentiviral (biosafety level 2 laboratory)
  • nucleofection (amaxa®)

Gene expression

  • qRT-PCR (TaqMan™)

Protein expression analysis

  • Western blot
  • immunoprecipitation
  • immunofluorescence microscopy

Protein localisation studies

  • confocal microscopy
  • immunohistochemistry

Analysis of protein function

  • Determination of the induction of apoptosis
  • Determination of the cell viability
  • Signal transduction analysis
  • Various reporter assays

Fee-for-service packages

Gene overexpression/knock-down:

  • Gene cloning (isolation of mRNA, cDNA synthesis, amplification and cloning of open reading frames)
  • Generation and application of viral and non-viral vectors to overexpress or knock-down genes in eukaryotic cell cultures as well as in primary cell cultures
  • Optimisation of viral transduction
  • Generation of stably expressing cell lines (polyclonal and/or monoclonal) in conjunction with expression analysis at the mRNA level and at the protein level, followed by functional characterisation
  • Development of improved, more physiological cell cultur models (co-cultures, cell stress/injury paradigms)
  • Overexpression and subsequent purification of proteins in laboratory scale (ca. 1 mg of protein) using bacterial and eukaryotic cell systems



Bayer Healthcare Pharmaceuticals, Hot Screen GmbH, Novalung GmbH, 4base lab GmbH, NMI TT GmbH


KIT (Karlsruhe Institute of Technology), University of Tübingen, University of Stuttgart