Protein Microarrays - A Promising Tool for Cancer Diagnosis

Protein Microarrays - A Promising Tool for Cancer Diagnosis
Schneiderhan-Marra N, Kirn A, Döttinger A, Templin M, Sauer G, Deissler H, Joos TO
Cancer Genomics & Proteomics. 2005 Jan; 2(1): 37-42.

Within recent years, protein microarrays have beendeveloped to quantify a large number of parameters present ina   given   sample   simultaneously.   Such   miniaturised   andparallelised sandwich immunoassays are of general interest forall  proteomic  and  diagnostic  approaches  in  which  severalparameters  have  to  be  determined  from  small  samples,  e.g.biopsy  material.  In  addition  to  planar  microarray-basedapproaches, bead-based flow cytometry is quite suitable for themultiplex  detection  of  target  molecules,  especially  when  alimited number of parameters are to be analysed. Appropriatesensitivity,    reproducibility    and    robustness    have    to    bedemonstrated  before  protein  microarray  technology  can  beused to characterise clinical samples and generate reliable datasets.  As  a  model  system  to  analyse  these  issues,  a  set  ofmultiplexed sandwich immunoassays based on Luminex beadswere  developed  to  screen  clinical  samples  for  the  presence  orabsence of marker proteins indicative of prognosis or responseto therapeutic options.