Increasing robustness and sensitivity of protein microarrays through microagitation and automation.

Increasing robustness and sensitivity of protein microarrays through microagitation and automation.
Hartmann M, Toegl A, Kirchner R, Templin MF, Joos TO
Anal Chim Acta. 2006 Mar 30;564(1):66-73. Epub 2006 Feb 21. DOI: 10.1016/j.aca.2006.01.041

Assay systems that employ protein microarrays for the analysis of complex samples are powerful tools to generate a high amount of data from a limiting amount of sample. Due to miniaturization, these systems are susceptible to fluctuations during signal generation and the use of uniform conditions for sample incubation and during the assay procedure is required to get reproducible results. Diffusion limits may prevent constant conditions on all parts of the array and can lead to the decease of the sensitivity of the array. Therefore, we set-up an automated assay system integrating a novel microagitation device using surface acoustic wave (SAW) technology. Multiplexed assays for the detection of autoantibodies from human serum and sandwich immunoassay for the detection of matrix metalloproteases (MMPs) were used to evaluate the system. Diffusion-rate limited solid phase reactions were enhanced by microagitation using the SAW technology resulting in up to three-fold higher signals. Pubmed