Expanding an efficient, electrically driven and CNT-tagged P450 system into the third dimension: a nanowired CNT containing and enzyme stabilising 3D sol-gel electrode

Although electrochemically catalysed P450 reactions have been described in literature previously, their efficiency and applicability remained limited. This is mostly due to low enzyme activity, laborious protein immobilisation techniques and the small electrode surface. We established a novel protein immobilisation for a determined orientation and electrical wiring of the enzyme without post-expressional modification. By introduction of an anchor-peptide at DNA level our method is applicable for medium to large size mutant libraries to be screened on and detected by an electrode system. The system was further expanded by using wired carbon nanotubes within a sol-gel matrix to create a three dimensional electrode.