A Bead-Based Multiplex Sandwich Immunoassay to Assess the Abundance and Posttranslational Modification State of beta-Catenin
Groll N, Sommersdorf C, Joos TO, Poetz O
Methods Mol Biol. 2015;1295:441-53. doi: 10.1007/978-1-4939-2550-6_31.
A system-wide analysis of cell signaling involves detecting and quantifying a range of proteins and their posttranslational modification states in the same cellular sample. We propose a protocol for a miniaturized, bead-based array and describe its efficiency in characterizing the different forms and functions of beta-catenin. The protocol provides detailed instructions for cell culture and bead array assays that enable insights into complex networks at the systems level.