DeepSee

Project Image:
  • Immunfluoreszenzfärbung eines patienten-abgeleiteten Mikrotumors.
Title of the project:

DeepSee

Teasertext:
Developing a single cell profiling platform for patient-derived microtumors
Project name:
DeepSee
Start:
01.04.2020
End:
30.09.2021
Project leader:
Dr. Christian Schmees
Project funding:
  • BMWi
Project management:
  • AiF Projekt GmbH
Funding reference number:
ZF4585505AJ9
Description:

Patient-derived microtumors (PDMs) and autologous immune cells will be isolated from fresh tumor tissue. PDM-immune cell co-cultures will be treated with chemo- and immunotherapeutics followed by single cell proteo-genomic analysis including custom algorithm generation to enable identification of treatment-induced changes of cellular PDM composition. Treated co-cultures will be characterized by flow cytometry, gene expression and cytokine analyses combined with next-generation sequencing based TCR analysis to re-identify TCRs prone to PDM infiltration. Bioinformatics tools including flow cytometry and drug-response data integration will be developed. A service for single cell PDM profiling including wet lab techniques and data analysis requirements will be jointly developed and commercialized.

Project partners:
  • Proteona Pte. Ltd, Singapur
  • Synovo GmbH, Tübingen