Macrophage retrieval from 3D biomaterials: A detailed comparison of common dissociation methods
Nora Feuerer, Johannes Morschl, Ruben Daum, Martin Weiss, Svenja Hinderer, Katja Schenke-Layland, Christopher Shipp
https://doi.org/10.1016/j.regen.2020.100035
The immune system is widely recognized as a crucial determinant in implant biocompatibility and tissue integration. In order to assess macrophage response to biomaterials, commonly used analysis techniques require the removal of cells from the material. This process inherently has a negative impact on the cells, but few studies have comprehensively compared different dissociation methods in terms of impact on cell yield, viability, phenotype and function.