A mesh microelectrode array for non-invasive electrophysiology within neural organoids

A mesh microelectrode array for non-invasive electrophysiology within neural organoids
Matthew McDonald; David Sebinger; Lisa Brauns; Laura Gonzalez-Cano; Yotam Menuchin-Lasowski; Michael Mierzejewski; Olympia-Ekaterini Psathaki; Angelika Stumpf; Jenny Wickham; Thomas Rauen; Hans Schöler; Peter D. Jones
Biosensors and Bioelectronics Volume 228; https://doi.org/10.1016/j.bios.2023.115223

Organoids are emerging in vitro models of human physiology. Neural models require the evaluation of functional activity of single cells and networks, which is commonly measured by microelectrode arrays. The characteristics of organoids clash with existing in vitro or in vivo microelectrode arrays. With inspiration from implantable mesh electronics and growth of organoids on polymer scaffolds, we fabricated suspended hammock-like mesh microelectrode arrays for neural organoids. We have demonstrated the growth of organoids enveloping these meshes and the culture of organoids on meshes for up to one year. Furthermore, we present proof-of-principle recordings of spontaneous electrical activity across the volume of an organoid. Our concept enables a new class of microelectrode arrays for in vitro models of three-dimensional electrically active tissue.