Rekombinante Proteinproduktion & Aufreinigung

Dr. Philipp Kaiser
BioDevCenter

+49 7121 51530-476

E-Mail

We offer comprehensive services for the production and purification of biological molecules – from enzymes and antibodies to antigens and nanobodies. Our expertise covers all steps from molecular biological design to the purified, quality-tested end product.

Our Services:

Enzyme Production

• We produce enzymes in suitable expression systems (e.g., E. coli or mammalian cells) and supply high-purity proteins for research, diagnostic, or development applications. Our production processes are scalable and flexibly tailored to your requirements.
• PNGaseRc for deglycosylation under acidic pH conditions can be ordered directly.

Antibody Production

We offer the production of recombinant antibodies in various formats. By using state-of-the-art expression systems, we ensure consistent quality and high purity.

Antigen Production

We produce high-quality recombinant antigens for diagnostic assays, screening procedures, or validation studies. Expression and purification are performed according to clearly defined quality standards to ensure optimal functionality.

Purification & Quality Control Services

Our purification strategies are individually tailored to the respective molecule and include multi-step chromatography procedures, depending on the requirements.

Each product is characterized using appropriate quality controls, such as:

• Purity analysis (e.g., SDS-PAGE, LC-MS, Endotox, HCP)
• Concentration determination
• Functional detection methods as required

Our Methods:

• Expression in E. coli, HEK, or CHO
• Affinity chromatography, ion-exchange chromatography, size exclusion chromatography, hydrophobic interaction chromatography
• Analytical methods: SDS-PAGE, LC-UV, LC-MS
• Octet® BLI binding studies

Our Devices:

• ÄKTA FPLC
• nanoTemper Panta
• Octet Red 96e