Curriculum vitae

Since 2011
Professor of Pharmaceutical Biotechnology at the University of Tübingen (Joint Professor with NMI)

2008 - 2011
Managing Director of the biotechnology company ChromoTek GmbH (Martinsried, Germany)

2008 - 2010
Junior Research Group Leader, Institute of Biology, LMU Munich

2006 - 2007
Project Leader "Chromobodies", Project Leader "Chromobodies", Institute of Biology, LMU Munich, Germany

2004 - 2005
Post-Doc, Institute of Biology, LMU Munich under Prof. Dr. Heinrich Leonhardt

2003 - 2004
Post-doctoral fellow, Institute for Academic Diabetes Research Hospital, Munich under PD Dr. Mathias Baucher

1999 - 2003
Dissertation (Dr. rer. nat.; PhD) at the Institute of Physical Chemistry, LMU Munich under Prof. Dr. Dr. Walter Neuwert: "Functional characterization of a mitochondrial mediate disease: The role of DDP1 and Tim13 for mitochondrial biogenesis".

1998
Diploma in Biology under Prof. Dr. Svante Pääbo: In vitro processing of overlapping tRNA transcripts in human mitochondria

1992 - 1998
Study of Biology at the Ludwig-Maximilians University of Munich

Prof. Dr. Ulrich Rothbauer

Head of Dept. Pharma and Biotech, Head of Recombinant Antibody Technology

Diplom-Biologe

T +49 (0)7121 51530-415

F +49 (0)7121 51530-16

E ulrich.rothbauer@nmi.de

Projects

Predictive diagnostics of individual immune responses in different diseases

Publications

A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies

Traenkle B, Segan S, Funmilayo F , Kaiser P, Rothbauer U

Scientific Reports, 10, (2020) 10:14267, https://doi.org/10.1038/s41598-020-71091-x
Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro

Segan S, Jakobi M, Khokhani P, Klimosch S, Billing F, Schneider M, Martin D, Metzger U, Biesemeier A, Xiong X, Mukherjee A, Steuer H, Keller B-M, Joos T, Schmolz M, Rothbauer U, Hartmann H, Burkhardt C, Lorenz G ,Schneiderhan-Marra N, and Shipp C

BioMed Research International, 2020, https://doi.org/10.1155/2020/3481549
A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells

Keller B, Maier J, Weldle M, Segan S, Traenkle B, Rothbauer U

Antibodies 2019, 8, 10; doi:10.3390/antib8010010
Chromobodies to quantify changes of endogenous protein concentration in living cells

Keller BM, Maier J, Secker KA, Egetemaier SM, Parfyonova Y, Rothbauer U, Traenkle B

Mol Cell Proteomics. 2018 Dec;17(12):2517-2533. doi: 10.1074/mcp.TIR118.000914. Epub 2018 Sep 18.
Speed up to find the right ones: rapid discovery of functional nanobodies

Rothbauer U

Nat Struct Mol Biol. 2018 Mar;25(3):199-201.
A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging

Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Vojnovic I, Pisak-Lukáts B, Endesfelder U, Rothbauer U

Nature Communications. 2018 Mar;9:930. DOI:10.1038/s41467-018-03191-2
Akt1 and Akt3 but not Akt2 through interaction with DNA-PKcs stimulate proliferation and post-irradiation cell survival of K-RAS-mutated cancer cells

Toulany M, Maier J, Iida M, Rebholz S, Holler M, Grottke A, Jüker M, Wheeler DL, Rothbauer U, Rodemann HP

Cell Death Discovery. 2017; 3:17072
Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy

Traenkle B, Rothbauer U

Front Immunol. 2017 Aug 24;8:1030. doi: 10.3389/fimmu.2017.01030. eCollection 2017.
A Nexus Consisting of Beta-Catenin and Stat3 Attenuates BRAF Inhibitor Efficacy and Mediates Acquired Resistance to Vemurafenib

Sinnberg T, Makino E, Krueger MA, Velic A, Macek B, Rothbauer U, Groll N, Potz O, Czemmel S, Niessner H, Meier F, Ikenberg K, Garbe C, Schittek B

EBioMedicine. 2016 Jun;8:132-49. doi: 10.1016/j.ebiom.2016.04.037. Epub 2016 May 1.
Role of BCL9L in transforming growth factor-beta (TGF-beta)-induced epithelial-to-mesenchymal-transition (EMT) and metastasis of pancreatic cancer

Sannino G, Armbruster N, Bodenhofer M, Haerle U, Behrens D, Buchholz M, Rothbauer U, Sipos B, Schmees C

Oncotarget. 2016 Oct 4; 7:73725-73738. doi: 10.18632/oncotarget.12455.
Nanobody Platform for Determination of Protein Structure, Application of Peptides and Proteins

Traenkle B, Kaiser PD, Rothbauer U

2016. in: Encyclopedia of Analytical Chemistry, John Wiley & Sons, Ltd, 2006.
From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors

Bauer SM, Kubiak JM, Rothbauer U, Laufer S

Methods Mol Biol. 2016;1360:123-48. doi: 10.1007/978-1-4939-3073-9_10.
A Multiplexed High-Content Screening Approach Using the Chromobody Technology to Identify Cell Cycle Modulators in Living Cells

Schorpp K, Rothenaigner I, Maier J, Traenkle B, Rothbauer U, Hadian K

J Biomol Screen. 2016 Oct;21(9):965-77. doi: 10.1177/1087057116641935. Epub 2016 Apr 4.
A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells

Buchfellner A, Yurlova L, Nuske S, Scholz AM, Bogner J, Ruf B, Zolghadr K, Drexler SE, Drexler GA., Girst S, Greubel C, Reindl J, Siebenwirth C, Romer T, Friedl AA, Rothbauer U

PLoS One. 2016 Mar 7;11(3):e0151041. doi: 10.1371/journal.pone.0151041. eCollection 2016.
Peptides in headlock - a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy

Braun MB, Traenkle B, Koch PA, Emele F, Weiss F, Poetz O, Stehle T, Rothbauer U

Sci Rep. 2016 Jan 21;6:19211. doi: 10.1038/srep19211.
Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies

Maier J, Traenkle B, Rothbauer U

Sci Rep. 2015 Aug 21;5:13402. doi: 10.1038/srep13402.
Live imaging of endogenous protein dynamics in zebrafish using chromobodies.

Panza P, Maier J, Schmees C, Rothbauer U, Söllner C.

Development. 2015 May 15;142(10):1879-84. doi: 10.1242/dev.118943.
Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells

Traenkle B, Emele F, Anton R, Poetz O, Haeussler RS, Maier J, Kaiser PD, Scholz AM, Nueske S, Buchfellner A, Romer T, Rothbauer U

Mol Cell Proteomics. 2015 Mar;14(3):707-23. doi: 10.1074/mcp.M114.044016. Epub 2015 Jan 16.
Generation of an alpaca-derived nanobody recognizing gamma-H2AX

Rajan M, Mortusewicz O, Rothbauer U, Hastert FD, Schmidthals K, Rapp A, Leonhardt H, Cardoso MC

FEBS Open Bio. 2015 Sep 21;5:779-88. doi: 10.1016/j.fob.2015.09.005. eCollection 2015.
Towards multiplexed protein-protein interaction analysis using protein tag-specific nanobodies

Groll N, Emele F, Poetz O, Rothbauer U

J Proteomics. 2015 Sep 8;127(Pt B):289-99. doi: 10.1016/j.jprot.2015.04.017. Epub 2015 May 1.
A p38 substrate-specific MK2-EGFP translocation assay for identification and validation of new p38 inhibitors in living cells: a comprising alternative for acquisition of cellular p38 inhibition data

Anton R, Bauer SM, Keck PR, Laufer S, Rothbauer U

PLoS One. 2014 Apr 17;9(4):e95641. doi: 10.1371/journal.pone.0095641. eCollection 2014.
Recent progress in generating intracellular functional antibody fragments to target and trace cellular components in living cells

Kaiser PD, Maier J, Traenkle B, Emele F, Rothbauer U

Biochim Biophys Acta. 2014 Nov;1844(11):1933-1942. doi: 10.1016/j.bbapap.2014.04.019. Epub 2014 May 2.
c-Jun/c-Fos heterodimers regulate cellular genes via a newly identified class of methylated DNA sequence motifs

Gustems M, Woellmer A, Rothbauer U, Eck SH, Wieland T, Lutter D, Hammerschmidt W

Nucleic Acids Res. 2014 Mar;42(5):3059-72. doi: 10.1093/nar/gkt1323. Epub 2013 Dec 25.
The Fluorescent Two-Hybrid Assay to Screen for Protein-Protein Interaction Inhibitors in Live Cells: Targeting the Interaction of p53 with Mdm2 and Mdm4

Yurlova L, Derks M, Buchfellner A, Hickson I, Janssen M, Morrison D, Stansfield I, Brown CJ, Ghadessy FJ, Lane DP, Rothbauer U, Zolghadr K, Krausz E

J Biomol Screen. 2014 Apr;19(4):516-25. doi: 10.1177/1087057113518067. Epub 2014 Jan 29.
A versatile assay for RNA-binding proteins in living cells

Strein C, Alleaume AM, Rothbauer U, Hentze MW, Castello A

RNA. 2014 May;20(5):721-31. doi: 10.1261/rna.043562.113. Epub 2014 Mar 24.

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