Prof. Dr. Ulrich Rothbauer

Stages of career

01/2018 - 12/2021 Head of Department PharmaBiotech at with the Natural and Medical Sciences Institute at the University of Tuebingen, NMI)
Since 11/2011 Professor for Pharmaceutical Biotechnology at the University of Tuebingen
(Joint Professorship with the Natural and Medical Sciences Institute at the University of Tuebingen, NMI)
2008 – 2011                 
CEO of the Biotech company ChromoTek GmbH (Martinsried)
2008 – 2010 Junior Group leader, GO-Bio, Biocenter, LMU
2006 – 2007 Project leader „Chromobodies“ Exist Seed Group, Biocenter, LMU
2004 – 2005 Postdoctoral Fellow in the lab of Prof. Dr Heinrich Leonhardt, Biocenter, LMU,
2003 – 2004 Postdoctoral Fellow in the lab of PD Dr. Mathias Bauer, Institute of Diabetic Research Academic Hospital, Munich



Prof. Dr. Ulrich Rothbauer
Groupleader Recombinant Antibody Technology


  • The interaction between anti-PF4 antibodies and anticoagulants in vaccine-induced thrombotic thrombocytopenia
    Singh, A., Toma, F., Uzun, G., Wagner, T., Pelzl, L., Zlamal, J., Freytag, V., Weich, K., Nowak-Hamau, S., Rothbauer, U., Althaus, K., Bakchoul, T.
  • A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1
    Fagbadebo, F., Kaiser, P., Zittlau, K., Bartlick, N., Wagner, T., Fröhlich, T., Jarjour, G., Nueske, S., Scholz, A., Tränkle, B., Macek, B., Rothbauer, U.
  • Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane
    Bellon J, Birkholz O, Richter C, Walter M, Kurre R, Piehler J, Eull F, Kenneweg H, Wilmes S, Rothbauer U, You C,
    Sotolongo Bello´ n et al., 2022, Cell Reports Methods 2, 100165 February 28, 2022 ª 2022 The Authors.
  • Decisive role of water and protein dynamics in residence time of p38α MAP kinase inhibitors
    Pantsar T, Kaiser P, Kudolo M, Forster M, Rothbauer U, Laufer S
    Nature Communications volume 13, Article number: 569 (2022)
  • Robust and durable serological response following pediatric SARS-CoV-2 infection
    Renk H, Dulovic A, Seidel A, Becker M, Fabricius D, Zernickel M, Junker D, Groß R, Müller J, Hilger A, Bode S, Fritsch L, Frieh P, Haddad A, Görne T, Remppis J, Ganzemueller T, Dietz A, Huzly D, Hengel H, Kaier K, Weber S, Jacobsen E, Kaiser P, Tränkle B, Rothbauer U, Stich M, Tönshoff B, Hoffmann G, Müller B, Ludwig C, Jahrsdörfer B, Schrezenmeier H, Peter A, Hörber S, Iftner T, Münch J, Stamminger T, Groß H, Wolkewitz M, Engel C, Liu W, Rizzi M, Hahn B, Henneke P, Franz A, Debatin K, Schneiderhan-Marra N, Janda A, Elling R
    Nature Communications volume 13, Article number: 128 (2022);
  • Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells
    Traenkle B, Kaiser PD, Pezzana S, Richardson J, Gramlich M, Wagner TR, Seyfried D, Weldle M, Holz S, Parfyonova Y, Nueske S, Scholz AM, Zeck A, Jakobi M, Schneiderhan-Marra N, Schaller M, Maurer A, Gouttefangeas C, Kneilling M, Pichler BJ, Sonanini D, Rothbauer U
    Front Immunol. 2021 Dec 9;12:799910. doi: 10.3389/fimmu.2021.799910. PMID: 34956237; PMCID: PMC8696186.
  • Nanobodies - Little helpers unravelling intracellular signaling
    Wagner TR, Rothbauer U
    Free Radic Biol Med. 2021 Nov 20;176:46-61. doi: 10.1016/j.freeradbiomed.2021.09.005. Epub 2021 Sep 15. PMID: 34536541.
  • Reduced serum neutralization capacity against SARS-CoV-2 variants in a multiplex ACE2 RBD competition assay
    Junker D, Dulovic A, Becker M, Wagner T R, Kaiser P D, Traenkle B, Rothbauer U, Kienzle K, Bunk S, Strümper C, Häberle H, Schmauder K, Malek N, Althaus K, Koeppen M, Bitzer M, Göpel S, Schneiderhan-Marra N
  • Diminishing immune responses against variants of concern in dialysis patients four months after SARS-CoV-2 mRNA vaccination
    Dulovic A, Strengert M, Morillas Ramos G, Becker M, Griesbaum J, Junker D, Lürken K, Beigel A, Wrenger E, Lonnemann G, Cossmann A, Stankov M, Dopfer-Jablonka A, Kaiser P, Traenkle B, Rothbauer U, Krause G, Schneiderhan-Marra N, Behrens G M.N.
  • A broadly neutralizing biparatopic Nanobody protects mice from lethal challenge with SARS-CoV-2 variants of concern
    Wagner T, Schnepf D, Beer J, Klingel K, Ruetalo N, Kaiser P, Junker D, Sauter M, raenkle B, Frecot D, Becker M, Schneiderhan-Marra N, Ohnemus A, Schwemmle M, Schindler M, Rothbauer U
    bioRxiv, doi:
  • Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers
    Nair A L , Mesch L, Schulz I, Becker H , Raible J, Kiessling H, Werner S, Rothbauer U , Schmees C, Busche M, Trennheuser S, Fricker G and Stelzle M
    Biosensors 2021, 11, 314.
  • Typically asymptomatic but with robust antibody formation: Children’s unique humoral immune response to SARS-CoV-2.
    Renk H, Dulovic A, Becker M, Fabricius D, Zernickel M, Junker D, Seidel A, Groß R, Hilger A, Bode S, Fritsch L, Frieh P, Haddad A, Görne T, Remppis J, Ganzemueller T, Dietz A, Huzly D, Hengel H, Kaier K, Weber S, Jacobsen EM, Kaise P, Traenkle B, Rothbauer U, Stich M, Tönshoff B, Hoffmann H, Müller B, Ludwig C, Jahrsdörfer B, Schrezenmeier H, Peter A, Hörber S, Iftner T, Münch J, Stamminger T, Groß HJ, Wolkewitz M, Engel C, Rizzi M, Henneke P, Franz AR, Debatin KM, Schneiderhan-Marra N, Janda A, Elling R
    medRxiv, doi:
  • Generation and characterization of the human induced pluripotent stem cell line NMIi010-A from peripheral blood mononuclear cells of a healthy 49–year old male individual
    Keller AK, Binner A, Breitmeyer R, Vogel S, Anderle N, Rothbauer U, Schenke-Layland K, Schmees C
    Stem Cell Research Volume 54, July 2021, 102427,
  • A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
    M.J.B. Matos, F. Trovão, J. Gonçalves, U. Rothbauer, M.G. Freire, A.M.J.B. Barbosa, A.S. Pina, A.C.A. Roque
  • Generation and characterization of the human induced pluripotent stem cell line NMIi010-A from peripheral blood mononuclear cells of a healthy 49–year old male individual
    Keller AL, Binner A, Breitmeyer R, Vogel S, Anderle N, Rothbauer U, Schenke-Layland K, Schmees C
    tem Cell Res. 2021 Jul;54:102427. doi: 10.1016/j.scr.2021.102427. Epub 2021 Jun 11. PMID: 34139596.
  • Immune response to SARS-CoV-2 variants of concern in vaccinated individuals
    Becker M, Dulovic A, Junker D, Ruetalo N, PKaiser PD, Pinilla YT, Heinzel C, Haering J, Traenkle B, Wagner TR, Layer M, Mehrlaender M, Mirakaj V, Held J, Planatscher H, Schenke-Layland K, Krause G, Strengert M, Bakchoul T, Althaus K, Fendel R, Kreidenweiss A, Koeppen M, Rothbauer U, Schindler M & Schneiderhan-Marra N
    NATURE COMMUNICATIONS| (2021) 12:3109 | |,;
  • NeutrobodyPlex - monitoring SARS-CoV-2 neutralizing immune responses using nanobodies
    Wagner TR, Ostertag E, Kaiser PD, Gramlich M, Ruetalo N, Junker D, Haering J, Traenkle B, Becker M, Dulovic A, Schweizer H, Nueske S, Scholz A, Zeck A, Schenke-Layland K, Nelde A, Strengert M, Walz JS, Zocher G, Stehle T, Schindler M, Schneiderhan-Marra N, Rothbauer U
    EMBO Rep. 2021 May 5;22(5):e52325. doi: 10.15252/embr.202052325. Epub 2021 Apr 27. PMID: 33904225; PMCID: PMC8097376.
  • HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1
    Gramlich M, Hays HCW, Crichton S, Kaiser PD, Heine A, Schneiderhan-Marra N, Rothbauer U, Stoll D, Maier S, Zeck A
    Antibodies (Basel). 2021 Mar 19;10(1):11. doi: 10.3390/antib10010011. PMID: 33808657; PMCID: PMC8006148. Copy Downloa
  • Multiplexed Serum Antibody Screening Platform Using Virus Extracts from Endemic Coronaviridae and SARS-CoV-2
    Fink S., Ruoff F., Stahl A., Becker M., Kaiser P., Traenkle B., Junker D., Weise F., Ruetalo N., Hörber S., Peter A., Nelde A., Walz j., Krause G., Baillot A., Schenke-Layland K., Joos TO., Rothbauer U., Schneiderhan-Marra N., Schindler M., Templin MF
    ACS Infectious Diseases 2021;
  • Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity
    Becker M, Strengert M, Junker D, Kaiser P, Kerrinnes T, Traenkle B, Dinter H, Häring J, Ghozzi S, Zeck A, Weise F, Peter A, Hörber S, Fink S, Ruoff F, Dulovic A, Bakchoul T, Baillot A, Lohse S, Cornberg M, Illig T, Gottlieb J, Smola S, Karch A, Berger K, Rammensee HG, Schenke-Layland K, Nelde A, Märklin M, Heitmann JS, Walz JS, Templin M, Joos TO, Rothbauer U, Krause K & Schneiderhan-Marra N
    NATURE COMMUNICATIONS | (2021) 12:1152 | |
  • Magnetic Bead-Based Immunoassay Allows Rapid, Inexpensive, and Quantitative Detection of Human SARS-CoV-2 Antibodies
    Huergo LF, Selim KA, Conzentino MS, Gerhardt ECM, Santos ARS, Wagner B, Alford JT, Deobald N, Pedrosa FO, de Souza EM, Nogueira MB, Raboni SM, Souto D, Rego FGM, Zanette DL, Aoki MN, Nardin JM, Fornazari B, Morales HMP, Borges VA, Nelde A, Walz JS, Becker M, Schneiderhan-Marra N, Rothbauer U, Reis RA, Forchhammer K
    ACS Sensors 2021, 6(3):703-708,
  • NeutrobodyPlex - Nanobodies to monitor a SARS-CoV-2 neutralizing immune response
    Wagner T., Ostertag E., Kaiser P., Gramlich M., Ruetalo N., Junker D., Haering J., Traenkle B., Becker M., Dulovic A., Schweizer H., Nueske S., Scholz A., Zeck A., Schenke-Layland K., Nelde A., Strengert M., Walz J., Zocher G., Stehle T., Schindler M., Schneiderhan-Marra N., Rothbauer U
    In EMBO Reports 2021; 22(5):e52325.
  • SARS-CoV-2-derived peptides define heterologous and COVID-19-induced T cell recognition
    Nelde A., Bilich T., Heitmann JS., Maringer Y., Salih HR., Roerden R., Luebke M., Bauer J., Rieth J., Wacker M., Peter A., Hörber S., Traenkle B., Kaiser PD., Rothbauer U., Becker M., Junker D., Krause G., Strengert M., Schneiderhan-Marra N., Joos TO., Kowalewski D.J., Stos-Zweifel V., Fehr M., Graf M., Gruber L., Rachfalski D., Preuß B., Hagelstein I., Märklin M., Bakchoul T., Gouttefangeas C., Kohlbacher O., Klein R., Stevanović S., Rammensee H.-G., Walz J.S.
    Nature Immunology 2021; 22:74–85, DOI:
  • A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies
    Traenkle B, Segan S, Funmilayo F , Kaiser P, Rothbauer U
    Scientific Reports, 10, (2020) 10:14267,
  • Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro
    Segan S, Jakobi M, Khokhani P, Klimosch S, Billing F, Schneider M, Martin D, Metzger U, Biesemeier A, Xiong X, Mukherjee A, Steuer H, Keller B-M, Joos T, Schmolz M, Rothbauer U, Hartmann H, Burkhardt C, Lorenz G ,Schneiderhan-Marra N, and Shipp C
    BioMed Research International, 2020,
  • A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells
    Keller B, Maier J, Weldle M, Segan S, Traenkle B, Rothbauer U
    Antibodies 2019, 8, 10; doi:10.3390/antib8010010
  • Chromobodies to quantify changes of endogenous protein concentration in living cells
    Keller BM, Maier J, Secker KA, Egetemaier SM, Parfyonova Y, Rothbauer U, Traenkle B
    Mol Cell Proteomics. 2018 Dec;17(12):2517-2533. doi: 10.1074/mcp.TIR118.000914. Epub 2018 Sep 18.
  • Speed up to find the right ones: rapid discovery of functional nanobodies
    Rothbauer U
    Nat Struct Mol Biol. 2018 Mar;25(3):199-201.
  • A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
    Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Vojnovic I, Pisak-Lukáts B, Endesfelder U, Rothbauer U
    Nature Communications. 2018 Mar;9:930. DOI:10.1038/s41467-018-03191-2
  • Akt1 and Akt3 but not Akt2 through interaction with DNA-PKcs stimulate proliferation and post-irradiation cell survival of K-RAS-mutated cancer cells
    Toulany M, Maier J, Iida M, Rebholz S, Holler M, Grottke A, Jüker M, Wheeler DL, Rothbauer U, Rodemann HP
    Cell Death Discovery. 2017; 3:17072
  • Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy
    Traenkle B, Rothbauer U
    Front Immunol. 2017 Aug 24;8:1030. doi: 10.3389/fimmu.2017.01030. eCollection 2017.
  • A Nexus Consisting of Beta-Catenin and Stat3 Attenuates BRAF Inhibitor Efficacy and Mediates Acquired Resistance to Vemurafenib
    Sinnberg T, Makino E, Krueger MA, Velic A, Macek B, Rothbauer U, Groll N, Potz O, Czemmel S, Niessner H, Meier F, Ikenberg K, Garbe C, Schittek B
    EBioMedicine. 2016 Jun;8:132-49. doi: 10.1016/j.ebiom.2016.04.037. Epub 2016 May 1.
  • Role of BCL9L in transforming growth factor-beta (TGF-beta)-induced epithelial-to-mesenchymal-transition (EMT) and metastasis of pancreatic cancer
    Sannino G, Armbruster N, Bodenhofer M, Haerle U, Behrens D, Buchholz M, Rothbauer U, Sipos B, Schmees C
    Oncotarget. 2016 Oct 4; 7:73725-73738. doi: 10.18632/oncotarget.12455.
  • Nanobody Platform for Determination of Protein Structure, Application of Peptides and Proteins
    Traenkle B, Kaiser PD, Rothbauer U
    2016. in: Encyclopedia of Analytical Chemistry, John Wiley & Sons, Ltd, 2006.
  • From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors
    Bauer SM, Kubiak JM, Rothbauer U, Laufer S
    Methods Mol Biol. 2016;1360:123-48. doi: 10.1007/978-1-4939-3073-9_10.
  • A Multiplexed High-Content Screening Approach Using the Chromobody Technology to Identify Cell Cycle Modulators in Living Cells
    Schorpp K, Rothenaigner I, Maier J, Traenkle B, Rothbauer U, Hadian K
    J Biomol Screen. 2016 Oct;21(9):965-77. doi: 10.1177/1087057116641935. Epub 2016 Apr 4.
  • A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells
    Buchfellner A, Yurlova L, Nuske S, Scholz AM, Bogner J, Ruf B, Zolghadr K, Drexler SE, Drexler GA., Girst S, Greubel C, Reindl J, Siebenwirth C, Romer T, Friedl AA, Rothbauer U
    PLoS One. 2016 Mar 7;11(3):e0151041. doi: 10.1371/journal.pone.0151041. eCollection 2016.
  • Peptides in headlock - a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy
    Braun MB, Traenkle B, Koch PA, Emele F, Weiss F, Poetz O, Stehle T, Rothbauer U
    Sci Rep. 2016 Jan 21;6:19211. doi: 10.1038/srep19211.
  • Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies
    Maier J, Traenkle B, Rothbauer U
    Sci Rep. 2015 Aug 21;5:13402. doi: 10.1038/srep13402.
  • Live imaging of endogenous protein dynamics in zebrafish using chromobodies.
    Panza P, Maier J, Schmees C, Rothbauer U, Söllner C.
    Development. 2015 May 15;142(10):1879-84. doi: 10.1242/dev.118943.
  • Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells
    Traenkle B, Emele F, Anton R, Poetz O, Haeussler RS, Maier J, Kaiser PD, Scholz AM, Nueske S, Buchfellner A, Romer T, Rothbauer U
    Mol Cell Proteomics. 2015 Mar;14(3):707-23. doi: 10.1074/mcp.M114.044016. Epub 2015 Jan 16.
  • Generation of an alpaca-derived nanobody recognizing gamma-H2AX
    Rajan M, Mortusewicz O, Rothbauer U, Hastert FD, Schmidthals K, Rapp A, Leonhardt H, Cardoso MC
    FEBS Open Bio. 2015 Sep 21;5:779-88. doi: 10.1016/j.fob.2015.09.005. eCollection 2015.
  • Towards multiplexed protein-protein interaction analysis using protein tag-specific nanobodies
    Groll N, Emele F, Poetz O, Rothbauer U
    J Proteomics. 2015 Sep 8;127(Pt B):289-99. doi: 10.1016/j.jprot.2015.04.017. Epub 2015 May 1.
  • A p38 substrate-specific MK2-EGFP translocation assay for identification and validation of new p38 inhibitors in living cells: a comprising alternative for acquisition of cellular p38 inhibition data
    Anton R, Bauer SM, Keck PR, Laufer S, Rothbauer U
    PLoS One. 2014 Apr 17;9(4):e95641. doi: 10.1371/journal.pone.0095641. eCollection 2014.
  • Recent progress in generating intracellular functional antibody fragments to target and trace cellular components in living cells
    Kaiser PD, Maier J, Traenkle B, Emele F, Rothbauer U
    Biochim Biophys Acta. 2014 Nov;1844(11):1933-1942. doi: 10.1016/j.bbapap.2014.04.019. Epub 2014 May 2.
  • c-Jun/c-Fos heterodimers regulate cellular genes via a newly identified class of methylated DNA sequence motifs
    Gustems M, Woellmer A, Rothbauer U, Eck SH, Wieland T, Lutter D, Hammerschmidt W
    Nucleic Acids Res. 2014 Mar;42(5):3059-72. doi: 10.1093/nar/gkt1323. Epub 2013 Dec 25.
  • The Fluorescent Two-Hybrid Assay to Screen for Protein-Protein Interaction Inhibitors in Live Cells: Targeting the Interaction of p53 with Mdm2 and Mdm4
    Yurlova L, Derks M, Buchfellner A, Hickson I, Janssen M, Morrison D, Stansfield I, Brown CJ, Ghadessy FJ, Lane DP, Rothbauer U, Zolghadr K, Krausz E
    J Biomol Screen. 2014 Apr;19(4):516-25. doi: 10.1177/1087057113518067. Epub 2014 Jan 29.
  • A versatile assay for RNA-binding proteins in living cells
    Strein C, Alleaume AM, Rothbauer U, Hentze MW, Castello A
    RNA. 2014 May;20(5):721-31. doi: 10.1261/rna.043562.113. Epub 2014 Mar 24.
  • Tris(hydroxymethyl)aminomethane Compatibility with N-Hydroxysuccinimide Ester Chemistry: Biotinylation of Peptides and Proteins in TRIS Buffer
    Kratzer U, Sommersdorf C, Maier S, Wagner TR, Templin M, Joos TO, Rothbauer U, Zeck A, Poetz O
    Bioconjug Chem. 2021 Sep 15;32(9):1960-1965. doi: 10.1021/acs.bioconjchem.1c00283. Epub 2021 Aug 18. PMID: 34406760.