Dr. Markus Templin

Forschungsgebiete 

  • Cellular signalling pathways
  • tumor biology
  • Biomarker identification and validation
  • assay development
  • miniaturized assay systems

Stages of career

since 2003  group leader assay development

2000 - today  NMI

1996 - 2000  Research associate, Max Planck Institute for Developmental Biology, Tübingen 

1994 - 1995  Postdoc, ICMB, University of Edinburgh, Scotland, UK

Dr. Markus Templin

Group Leader Assay Development
Diplom-Biologe
T +49 (0)7121 51530-828
F +49 (0)7121 51530-16

Publications

  • Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3D cultures
    Schumacher D, Andrieux G, Boehnke K, Keil M, Silvestri A, Silvestrov M, Keilholz U, Haybaeck J, Erdmann G, Sachse C, Templin M, Hoffmann J, Boerries M, Schäfer R, Regenbrecht C
    PLoS Genet 15(3): e1008076.
  • Mouse Hepatomas with Ha-ras and B-raf Mutations Differ in Mitogen-Activated Protein Kinase Signaling and Response to Constitutive Androstane Receptor Activation
    Braeuning A, Kollotzek F, Zeller E, Knorpp T, Templin MF, Schwarz M
    Drug Metab Dispos. 2018 Nov;46(11):1462-1465. doi: 10.1124/dmd.118.083014. Epub 2018 Aug 16.
  • A high-throughput pipeline for validation of antibodies
    Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
    Nat Methods. 2018 Nov;15(11):909-912. doi: 10.1038/s41592-018-0179-8. Epub 2018 Oct 30.
  • Peptide-Based Sandwich Immunoassay for the Quantification of the Membrane Transporter Multidrug Resistance Protein
    Poetz O, Dieze T, Hammer H, Weiß F, Sommersdorf C, Templin MF, Esdar C, Zimmermann A, Stevanovic S, Bedke J, Stenzl A, Joos TO.
    Anal Chem. 2018 May 1;90(9):5788-5794. doi: 10.1021/acs.analchem.8b00152. Epub 2018 Apr 12.
  • Epidermal Growth Factor Represses Constitutive Androstane Receptor Expression in Primary Human Hepatocytes and Favors Regulation by Pregnane X Receptor.
    de Boussac H, Gondeau C, Briolotti P, Duret C, Treindl F, Römer M, Fabre JM, Herrero A, Ramos J, Maurel P, Templin M, Gerbal-Chaloin S, Daujat-Chavanieu M
    Drug Metab Dispos. 2018 Mar;46(3):223-236. doi: 10.1124/dmd.117.078683. Epub 2017 Dec 21.
  • Structure-dependent induction of apoptosis by hepatotoxic pyrrolizidine alkaloids in the human hepatoma cell line HepaRG: Single versus repeated exposure
    Waizenegger J, Braeuning A, Templin M, Lampen A, Hessel-Pras S
    Food Chem Toxicol. 2018 Apr;114:215-226. doi: 10.1016/j.fct.2018.02.036. Epub 2018 Feb 16.
  • Platelet protein biomarker panel for ovarian cancer diagnosis
    Lomnytska M, Pinto R, Becker S, Engström U, Gustafsson S, Björklund C, Templin M, Bergstrand J, Xu L, Widengren J, Epstein E, Franzén B, Auer G.
    Biomark Res. 2018 Jan 12;6:2. doi: 10.1186/s40364-018-0118-y.
  • Impact of serum and dialysates obtained from chronic hemodialysis patients maintained on high cut-off membranes on inflammation profile in human THP-1 monocytes
    Trojanowicz B, Ulrich C, Fiedler R, Storr M, Boehler T, Martus P, Pawlak M, Glomb MA, Henning C, Templin M, Werner K, Zickler D, Willy K, Schindler R, Girndt M
    Hemodial Int. 2017 Jul;21(3):348-358. doi: 10.1111/hdi.12494. Epub 2016 Sep 26.
  • Miniaturized Multiplexed Protein Binding Assays
    Templin MF, Poetz O, Schwenk JM, Stoll D, Joos TO
    W.-L. Xing and J. Cheng. Frontiers in Biochip Technology. Boston, MA: Springer US; 2006. 61-87.
  • Medium Cut-Off (MCO) Membranes Reduce Inflammation in Chronic Dialysis Patients-A Randomized Controlled Clinical Trial
    Zickler D, Schindler R, Willy K, Martus P, Pawlak M, Storr M, Hulko M, Boehler T, Glomb MA, Liehr K, Henning C, Templin M, Trojanowicz B, Ulrich C, Werner K, Fiedler R, Girndt M
    PLoS One. 2017 Jan 13;12(1):e0169024. doi: 10.1371/journal.pone.0169024. eCollection 2017.
  • Coordinating Role of RXRalpha in Downregulating Hepatic Detoxification during Inflammation Revealed by Fuzzy-Logic Modeling
    Keller R, Klein M, Thomas M, Drager A, Metzger U, Templin MF, Joos TO, Thasler WE, Zell A, Zanger UM
    PLoS Comput Biol. 2016 Jan 4;12(1):e1004431. doi: 10.1371/journal.pcbi.1004431. eCollection 2016 Jan.
  • High cut-off dialysis in chronic haemodialysis patients
    Girndt M, Fiedler R, Martus P, Pawlak M, Storr M, Bohler T, Glomb MA, Liehr K, Henning C, Templin M, Trojanowicz B, Ulrich C, Werner K, Zickler D, Schindler R
    Eur J Clin Invest. 2015 Dec;45(12):1333-40. doi: 10.1111/eci.12559.
  • RPPApipe: a pipeline for the analysis of reverse-phase protein array data
    Eichner J, Heubach Y, Ruff M, Kohlhof H, Strobl S, Mayer B, Pawlak M, Templin MF
    Biosystems. 2014 Aug;122:19-24. doi: 10.1016/j.biosystems.2014.06.009. Epub 2014 Jun 18.
  • A bead-based western for high-throughput cellular signal transduction analyses
    Treindl F, Ruprecht B, Beiter Y, Schultz S, Dottinger A, Staebler A, Joos TO, Kling S, Poetz O, Fehm T, Neubauer H, Kuster B, Templin MF
    Nat Commun. 2016 Sep 23;7:12852. doi: 10.1038/ncomms12852.
  • EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer
    Schneck H, Gierke B, Uppenkamp F, Behrens B, Niederacher D, Stoecklein NH, Templin MF, Pawlak M, Fehm T, Neubauer H
    PLoS One. 2015 Dec 22;10(12):e0144535. doi: 10.1371/journal.pone.0144535. eCollection 2015.
  • Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry
    Weiß F, Schnabel A, Planatscher H, van den Berg BHJ, Serschnitzki B, Nuessler AK, Thasler WE, Weiss TS, Reuss M, Stoll D, Templin MF, Joos TO, Marcus K, Poetz O
    Sci Rep. 2015 Mar 4;5:8759. doi: 10.1038/srep08759.
  • Bead-Based Peptide Arrays for Profiling the Specificity of Modification State-Specific Antibodies
    Filomena A, Beiter Y, Templin MF, Joos TO, Schneiderhan-Marra N, Poetz O
    Methods Mol Biol. 2015;1348:251-65. doi: 10.1007/978-1-4939-2999-3_22.
  • Cross-platform toxicogenomics for the prediction of non-genotoxic hepatocarcinogenesis in rat
    Romer M, Eichner J, Metzger U, Templin MF, Plummer S, Ellinger-Ziegelbauer H, Zell A
    PLoS One. 2014 May 15;9(5):e97640. doi: 10.1371/journal.pone.0097640. eCollection 2014.
  • Ha-ras and β-Catenin oncoproteins orchestrate metabolic programs in mouse liver tumors
    Unterberger EB, Eichner J, Wrzodek C, Lempiäinen H, Luisier R, Terranova R, Metzger U, Plummer S, Knorpp T, Braeuning A, Moggs J, Templin MF, Honndorf V, Piotto M, Zell A, Schwarz M
    International Journal of Cancer. 2014 Oct 1; 135(7): 1574-1585. https://doi.org/10.1002/ijc.28798
  • Multiple Protein Analysis of Formalin-fixed and Paraffin-embedded Tissue Samples with Reverse phase Protein Arrays
    Assadi M, Lamerz J, Jarutat T, Farfsing A, Paul H, Gierke B, Breitinger E, Templin MF, Essioux L, Arbogast S, Venturi M, Pawlak M, Langen H, Schindler T
    Mol Cell Proteomics. 2013 Sep;12(9):2615-22. doi: 10.1074/mcp.M112.023051. Epub 2013 May 7.
  • Multiple protein analysis of FFPE tissue samples with reverse-phase protein arrays
    Assadi M, Lamerz J, Jarutat T, Farfsing A, Paul H, Gierke B, Breitinger E, Templin MF, Essioux L, Arbogast S, Venturi M, Pawlak M, Langen H, Schindler T
    Mol Cell Proteomics. 2013 Sep;12(9):2615-22. doi: 10.1074/mcp.M112.023051. Epub 2013 May 7.
  • From spots to beads-PTM-peptide bead arrays for the characterization of anti-histone antibodies
    Heubach Y, Planatscher H, Sommersdorf C, Maisch D, Maier J, Joos TO, Templin MF, Poetz O
    Proteomics. 2013 Mar;13(6):1010-5. doi: 10.1002/pmic.201200383.
  • Identification of Dlk1-Dio3 imprinted gene cluster noncoding RNAs as novel candidate biomarkers for liver tumor promotion
    Lempiainen H, Couttet P, Bolognani F, Muller A, Dubost V, Luisier R, Del Rio Espinola A, Vitry V, Unterberger EB, Thomson JP, Treindl F, Metzger U, Wrzodek C, Hahne F, Zollinger T, Brasa S, Kalteis M, Marcellin M, Giudicelli F, Braeuning A, Morawiec L, Zamurovic N, Langle U, Scheer N, Schubeler D, Goodman J, Chibout SD, Marlowe J, Theil D, Heard DJ, Grenet O, Zell A, Templin MF, Meehan RR, Wolf RC, Elcombe CR, Schwarz M, Moulin P, Terranova R, Moggs JG
    Toxicol Sci. 2013 Feb;131(2):375-86. doi: 10.1093/toxsci/kfs303. Epub 2012 Oct 22.
  • Combining ultracentrifugation and peptide termini group-specific immunoprecipitation for multiplex plasma protein analysis
    Volk S, Schreiber TD, Eisen D, Wiese C, Planatscher H, Pynn CJ, Stoll D, Templin MF, Joos TO, Poetz O
    Mol Cell Proteomics. 2012 Jul;11(7):O111.015438. Epub 2012 Apr 23.
  • A Comprehensive Small Interfering RNA Screen Identifies Signaling Pathways Required for Gephyrin Clustering
    Wuchter J, Beuter S, Treindl F, Herrmann T, Zeck G, Templin MF, Volkmer H
    The Journal of Neuroscience. 2012 Oct 17; 32(42):14821–14834
  • Sequential multiplex analyte capturing for phospho-protein profiling
    Poetz O, Henzler T, Hartmann M, Kazmaier C, Templin MF, Herget T, Joos TO
    Mol Cell Proteomics. 2010 Nov;9(11):2474-81. Epub 2010 Aug 3. doi: 10.1074/mcp.M110.002709
  • Minimum information about a protein affinity reagent (MIAPAR)
    Bourbeillon J, Orchard S, Benhar I, Borrebaeck C, Templin M, de Daruvar A, Dubel S, Frank R, Gibson F, Gloriam D, Haslam N, Hiltker T, Humphrey-Smith I, Hust M, Juncker D, Koegl M, Konthur Z, Korn B, et al.
    NatBiotechnol. 2010 Jul;28(7):650-3. doi: 10.1038/nbt0710-650.
  • Targeting peptide termini - a novel immunoaffinity approach to reduce complexity in mass spectrometric protein identification
    Hoeppe S, Schreiber TD, Planatscher H, Zell A, Templin MF, Stoll D, Joos TO, Poetz O
    Mol Cell Proteomics. 2011 Feb;10(2):M110.002857. doi: 10.1074/mcp.M110.002857. Epub 2010 Oct 20.
  • Sequential multiplex analyte capturing for phosphor-protein profiling
    Poetz O, Henzler T, Hartmann M, Kazmaier C, Templin MF, Herget T, Joos TO
    Mol Cell Proteomics. 2010 Nov; 9(11): 2474–2481. Published online 2010 Aug 3. doi: 10.1074/mcp.M110.002709
  • Optimal selection of epitopes for TXP-immunoaffinity mass spectrometry
    Planatscher H, Supper J, Poetz O, Stoll D, Joos T, Templin MF, Zell A
    Algorithms Mol Biol. 2010 Jun 25;5:28. doi: 10.1186/1748-7188-5-28
  • Protein microarrays for diagnostic assays.
    Hartmann M, Roeraade J, Stoll D, Templin MF, Joos TO
    Anal Bioanal Chem. 2009 Mar;393(5):1407-16. Epub 2008 Sep 20. doi: 10.1007/s00216-008-2379-z.
  • On display on a bug: a systematic approach to characterize antibodies
    Knorpp T, Templin MF
    Nat Methods. 2008 Dec;5(12):1003-4. DOI: 10.1038/nmeth1208-1003
  • Novel functional profiling approach combining reverse phase protein microarrays and human 3-D ex vivo tissue cultures: expression of apoptosis-related proteins in human colon cancer.
    Pirnia F, Pawlak M, Thallinger GG, Gierke B, Templin MF, Kappeler A, Betticher DC, Gloor B, Borner MM
    Proteomics. 2009 Jul;9(13):3535-48. doi: 10.1002/pmic.200800159.
  • Proteome wide screening using peptide affinity capture
    Poetz O, Hoeppe S, Templin MF, Stoll D, Joos TO
    Proteomics. 2009 Mar;9(6):1518-23. doi: 10.1002/pmic.200800842.
  • Tumor promotion in liver of mice with a conditional Cx26 knockout.
    Marx-Stoelting P, Mahr J, Knorpp T, Schreiber S, Templin MF, Ott T, Buchmann A, Schwarz M
    Toxicol Sci. 2008 Jun;103(2):260-7. Epub 2008 Feb 27. doi: 10.1093/toxsci/kfn043
  • Expanding assay dynamics: a combined competitive and direct assay system for the quantification of proteins in multiplexed immunoassays
    Hartmann M, Schrenk M, Dottinger A, Nagel S, Roeraade J, Joos TO, Templin MF
    Clin Chem. 2008 Jun;54(6):956-63. Epub 2008 Apr 24. doi: 10.1373/clinchem.2007.099812
  • Increasing robustness and sensitivity of protein microarrays through microagitation and automation.
    Hartmann M, Toegl A, Kirchner R, Templin MF, Joos TO
    Anal Chim Acta. 2006 Mar 30;564(1):66-73. Epub 2006 Feb 21. DOI: 10.1016/j.aca.2006.01.041
  • Protein Microarrays - Neue Systeme für die Proteomforschung
    Joos TO, Pawlak M, Templin M, Stoll D
    GIT Labor-Fachzeitschrift. 2006; 10, page 890-892.
  • Protein microarrays: applications and future challenges
    Stoll D, Templin MF, Bachmann J, Joos TO
    Curr Opin Drug Discov Devel. 2005 Mar;8(2):239-52.
  • Protein Microarrays - A Promising Tool for Cancer Diagnosis
    Schneiderhan-Marra N, Kirn A, Döttinger A, Templin M, Sauer G, Deissler H, Joos TO
    Cancer Genomics & Proteomics. 2005 Jan; 2(1): 37-42.
  • Protein microarrays: catching the proteome.
    Poetz O, Schwenk JM, Kramer S, Stoll D, Templin MF, Joos TO
    Mech Ageing Dev. 2005 Jan;126(1):161-70. DOI: 10.1016/j.mad.2004.09.030
  • Protein microarrays for antibody profiling: Specificity and affinity determination on a chip.
    Poetz O, Ostendorp R, Brocks B, Schwenk JM, Stoll D, Joos TO, Templin MF
    Proteomics. 2005 Jun;5(9):2402-11. DOI: 10.1002/pmic.200401299
  • Protein microarrays and multiplexed sandwich immunoassays: what beats the beads?
    Templin MF, Stoll D, Bachmann J, Joos TO
    Comb Chem High Throughput Screen. 2004 May;7(3):223-9.
  • Microarray technology: an increasing variety of screening tools for proteomic research.
    Stoll D, Bachmann J, Templin MF, Joos TO
    DDT:Targets. 2004 Dec 15; 9(24): 10-7
  • Protein microarrays: promising tools for proteomic research
    Templin MF, Stoll D, Schwenk JM, Pötz O, Kramer S, Joos TO
    Proteomics. 2003 Nov;3(11):2155-66.
  • Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3D cultures
    Schumacher D, Andrieux G, Boehnke K, Keil M, Silvestri A, Silvestrov M, Keilholz U, Haybaeck J, Erdmann G, Sachse C, Templin M, Hoffmann J, Boerries M, Schafer R, Regenbrecht CRA
    PLoS Genet 15(3): e1008076. https://doi.org/10.1371/journal.pgen.1008076