Development of improved cell culture models and methods for gene transfer
Fields of activity
We focus on the analysis of cellular responses to gene expression in order to validate target genes for the pharmaceutical industry. Furthermore, we aim at developing improved cell culture models (co-culture, inflammatory response to exposure to hazardous substances), which, due to their more physiological nature, allow higher predictivity.
since 2009 Group leader of Target Expressions Systems
2002-2009 Head of laboratory in the Dept. of Molecular Biology
1998-2002 Post-doctoral fellow at the Max Planck Institute of Biology, Tübingen
1997 Doctoral degree at the Free University of Berlin (bacteriophage DNA replication)
1994-1996 Doctoral fellow at the National Centre of Biotechnology, Madrid, Spain
1987-1993 Student of biochemistry at the Free University of Berlin
Dr. Frank Weise
A touch of glue to complete bacteriophage assembly: the Tail-to-Head Joining Protein (THJP) familyAuzat I, Petitpas I, Lurz R, Weise F, Tavares PMol Microbiol. 2014 Jan 21;91(6): 1164-1178. doi: 10.1111/mmi.12526.
A New In Vitro Model to Study Cellular Responses after Thermomechanical Damage in Monolayer CulturesHettler A, Werner S, Eick S, Laufer S, Weise FPLoS One. 2013; 8(12): e82635. doi: 10.1371/journal.pone.0082635
Cell-based microarrays of infectious adenovirus encoding short hairpin RNA (shRNA)Volkmer H, Weise FMethods Mol Biol. 2011;706:97-106.
A novel reverse transduction adenoviral array for the functional analysis of shRNA librariesOehmig A, Klotzbucher A, Thomas M, Weise F, Hagner U, Brundiers R, Waldherr D, Lingnau A, Knappik A, Kubbutat, MH, Joos TO, Volkmer HBMC Genomics. 2008 Sep 24;9:441. doi: 10.1186/1471-2164-9-441.
Kinetics of endocytosis and recycling of the GPI-anchored variant surface glycoprotein in Trypanosoma brucei.Engstler M, Thilo L, Weise F, Grunfelder CG, Schwarz H, Boshart M, Overath PJ Cell Sci. 2004 Mar 1;117(Pt 7):1105-15. DOI: 10.1242/jcs.00938
Endocytosis of a glycosylphosphatidylinositol-anchored protein via clathrin-coated vesicles, sorting by default in endosomes, and exocytosis via RAB11-positive carriers.Grunfelder CG, Engstler M, Weise F, Schwarz H, Stierhof YD, Morgan GW, Field MC, Overath PMol Biol Cell. 2003 May;14(5):2029-40. Epub 2003 Feb 6. doi: 10.1091/mbc.E02-10-0640