Dr. Björn Tränkle

Areas of activity

Preclinical development of biologics, Discovery, production, functionalization and cellular testing of remobinant binding molecules (anitbodies, anitbody-derivatives, nanobodies) for diagnostics molecular imaging (PET imaging) and therapeutic immunomodulation

Stages of career

2020 - present  Scientist at Recombinant Antibody Technologies Group with Prof. Dr. Ulrich Rothbauer

2013 - 2020  Postdoc at University of Tuebingen, Pharmaceutical Biotechnology Group with Prof. Dr. Ulrich Rothbauer

2010 -2013  Dissertation (Dr. rer. nat.; PhD) at NMI / Universtiy of Tuebingen with Prof. Dr. Michael Schwarz and Prof. Dr. Stefan Laufer: "The Role of PLexin A4 in the regulation of proliferation and apoptosis of Prostate Cancer Cells".

2009  Diploma Thesis in Biochemistry at NMI, Molecular Biology Groupd with Frank Weise: "Suitability analysis of the proteins G2E3, CCAR1, GREB1 and Nur77 as biomarker or therapeutic target for oncotherapy"

2002 - 2009  Study of Biochemistry at the Eberhard Karls University in Tuebingen and Montana State University in Bozeman, USA (2005 - 2006)
Dr. Björn Tränkle
Diplom-Biochemiker

Publications

  • A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1
    Fagbadebo, F., Kaiser, P., Zittlau, K., Bartlick, N., Wagner, T., Fröhlich, T., Jarjour, G., Nueske, S., Scholz, A., Tränkle, B., Macek, B., Rothbauer, U.
    https://doi.org/10.3389/fmolb.2022.835302
  • Robust and durable serological response following pediatric SARS-CoV-2 infection
    Renk H, Dulovic A, Seidel A, Becker M, Fabricius D, Zernickel M, Junker D, Groß R, Müller J, Hilger A, Bode S, Fritsch L, Frieh P, Haddad A, Görne T, Remppis J, Ganzemueller T, Dietz A, Huzly D, Hengel H, Kaier K, Weber S, Jacobsen E, Kaiser P, Tränkle B, Rothbauer U, Stich M, Tönshoff B, Hoffmann G, Müller B, Ludwig C, Jahrsdörfer B, Schrezenmeier H, Peter A, Hörber S, Iftner T, Münch J, Stamminger T, Groß H, Wolkewitz M, Engel C, Liu W, Rizzi M, Hahn B, Henneke P, Franz A, Debatin K, Schneiderhan-Marra N, Janda A, Elling R
    Nature Communications volume 13, Article number: 128 (2022); https://doi.org/10.1038/s41467-021-27595-9
  • Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells
    Traenkle B, Kaiser PD, Pezzana S, Richardson J, Gramlich M, Wagner TR, Seyfried D, Weldle M, Holz S, Parfyonova Y, Nueske S, Scholz AM, Zeck A, Jakobi M, Schneiderhan-Marra N, Schaller M, Maurer A, Gouttefangeas C, Kneilling M, Pichler BJ, Sonanini D, Rothbauer U
    Front Immunol. 2021 Dec 9;12:799910. doi: 10.3389/fimmu.2021.799910. PMID: 34956237; PMCID: PMC8696186.
  • Diminishing immune responses against variants of concern in dialysis patients four months after SARS-CoV-2 mRNA vaccination
    Dulovic A, Strengert M, Morillas Ramos G, Becker M, Griesbaum J, Junker D, Lürken K, Beigel A, Wrenger E, Lonnemann G, Cossmann A, Stankov M, Dopfer-Jablonka A, Kaiser P, Traenkle B, Rothbauer U, Krause G, Schneiderhan-Marra N, Behrens G M.N.
    medRxiv, https://doi.org/10.1101/2021.08.16.21262115
  • Typically asymptomatic but with robust antibody formation: Children’s unique humoral immune response to SARS-CoV-2.
    Renk H, Dulovic A, Becker M, Fabricius D, Zernickel M, Junker D, Seidel A, Groß R, Hilger A, Bode S, Fritsch L, Frieh P, Haddad A, Görne T, Remppis J, Ganzemueller T, Dietz A, Huzly D, Hengel H, Kaier K, Weber S, Jacobsen EM, Kaise P, Traenkle B, Rothbauer U, Stich M, Tönshoff B, Hoffmann H, Müller B, Ludwig C, Jahrsdörfer B, Schrezenmeier H, Peter A, Hörber S, Iftner T, Münch J, Stamminger T, Groß HJ, Wolkewitz M, Engel C, Rizzi M, Henneke P, Franz AR, Debatin KM, Schneiderhan-Marra N, Janda A, Elling R
    medRxiv, doi: https://doi.org/10.1101/2021.07.20.21260863
  • Immune response to SARS-CoV-2 variants of concern in vaccinated individuals
    Becker M, Dulovic A, Junker D, Ruetalo N, PKaiser PD, Pinilla YT, Heinzel C, Haering J, Traenkle B, Wagner TR, Layer M, Mehrlaender M, Mirakaj V, Held J, Planatscher H, Schenke-Layland K, Krause G, Strengert M, Bakchoul T, Althaus K, Fendel R, Kreidenweiss A, Koeppen M, Rothbauer U, Schindler M & Schneiderhan-Marra N
    NATURE COMMUNICATIONS| (2021) 12:3109 | https://doi.org/10.1038/s41467-021-23473-6 | www.nature.com/naturecommunications11234567890():,;
  • NeutrobodyPlex - monitoring SARS-CoV-2 neutralizing immune responses using nanobodies
    Wagner TR, Ostertag E, Kaiser PD, Gramlich M, Ruetalo N, Junker D, Haering J, Traenkle B, Becker M, Dulovic A, Schweizer H, Nueske S, Scholz A, Zeck A, Schenke-Layland K, Nelde A, Strengert M, Walz JS, Zocher G, Stehle T, Schindler M, Schneiderhan-Marra N, Rothbauer U
    EMBO Rep. 2021 May 5;22(5):e52325. doi: 10.15252/embr.202052325. Epub 2021 Apr 27. PMID: 33904225; PMCID: PMC8097376.
  • Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity
    Becker M, Strengert M, Junker D, Kaiser P, Kerrinnes T, Traenkle B, Dinter H, Häring J, Ghozzi S, Zeck A, Weise F, Peter A, Hörber S, Fink S, Ruoff F, Dulovic A, Bakchoul T, Baillot A, Lohse S, Cornberg M, Illig T, Gottlieb J, Smola S, Karch A, Berger K, Rammensee HG, Schenke-Layland K, Nelde A, Märklin M, Heitmann JS, Walz JS, Templin M, Joos TO, Rothbauer U, Krause K & Schneiderhan-Marra N
    NATURE COMMUNICATIONS | (2021) 12:1152 | https://doi.org/10.1038/s41467-021-20973-3 | www.nature.com/naturecommunications
  • NeutrobodyPlex - Nanobodies to monitor a SARS-CoV-2 neutralizing immune response
    Wagner T., Ostertag E., Kaiser P., Gramlich M., Ruetalo N., Junker D., Haering J., Traenkle B., Becker M., Dulovic A., Schweizer H., Nueske S., Scholz A., Zeck A., Schenke-Layland K., Nelde A., Strengert M., Walz J., Zocher G., Stehle T., Schindler M., Schneiderhan-Marra N., Rothbauer U
    In EMBO Reports 2021; 22(5):e52325. https://doi.org/10.15252/embr.202052325
  • SARS-CoV-2-derived peptides define heterologous and COVID-19-induced T cell recognition
    Nelde A., Bilich T., Heitmann JS., Maringer Y., Salih HR., Roerden R., Luebke M., Bauer J., Rieth J., Wacker M., Peter A., Hörber S., Traenkle B., Kaiser PD., Rothbauer U., Becker M., Junker D., Krause G., Strengert M., Schneiderhan-Marra N., Joos TO., Kowalewski D.J., Stos-Zweifel V., Fehr M., Graf M., Gruber L., Rachfalski D., Preuß B., Hagelstein I., Märklin M., Bakchoul T., Gouttefangeas C., Kohlbacher O., Klein R., Stevanović S., Rammensee H.-G., Walz J.S.
    Nature Immunology 2021; 22:74–85, DOI: https://doi.org/10.1038/s41590-020-00808-x
  • A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies
    Traenkle B, Segan S, Funmilayo F , Kaiser P, Rothbauer U
    Scientific Reports, 10, (2020) 10:14267, https://doi.org/10.1038/s41598-020-71091-x
  • A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells
    Keller B, Maier J, Weldle M, Segan S, Traenkle B, Rothbauer U
    Antibodies 2019, 8, 10; doi:10.3390/antib8010010
  • Chromobodies to quantify changes of endogenous protein concentration in living cells
    Keller BM, Maier J, Secker KA, Egetemaier SM, Parfyonova Y, Rothbauer U, Traenkle B
    Mol Cell Proteomics. 2018 Dec;17(12):2517-2533. doi: 10.1074/mcp.TIR118.000914. Epub 2018 Sep 18.
  • A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
    Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Vojnovic I, Pisak-Lukáts B, Endesfelder U, Rothbauer U
    Nature Communications. 2018 Mar;9:930. DOI:10.1038/s41467-018-03191-2
  • Under the Microscope: Single-Domain Antibodies for Live-Cell Imaging and Super-Resolution Microscopy
    Traenkle B, Rothbauer U
    Front Immunol. 2017 Aug 24;8:1030. doi: 10.3389/fimmu.2017.01030. eCollection 2017.
  • Nanobody Platform for Determination of Protein Structure, Application of Peptides and Proteins
    Traenkle B, Kaiser PD, Rothbauer U
    2016. in: Encyclopedia of Analytical Chemistry, John Wiley & Sons, Ltd, 2006.
  • A Multiplexed High-Content Screening Approach Using the Chromobody Technology to Identify Cell Cycle Modulators in Living Cells
    Schorpp K, Rothenaigner I, Maier J, Traenkle B, Rothbauer U, Hadian K
    J Biomol Screen. 2016 Oct;21(9):965-77. doi: 10.1177/1087057116641935. Epub 2016 Apr 4.
  • Peptides in headlock - a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy
    Braun MB, Traenkle B, Koch PA, Emele F, Weiss F, Poetz O, Stehle T, Rothbauer U
    Sci Rep. 2016 Jan 21;6:19211. doi: 10.1038/srep19211.
  • Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells
    Traenkle B, Emele F, Anton R, Poetz O, Haeussler RS, Maier J, Kaiser PD, Scholz AM, Nueske S, Buchfellner A, Romer T, Rothbauer U
    Mol Cell Proteomics. 2015 Mar;14(3):707-23. doi: 10.1074/mcp.M114.044016. Epub 2015 Jan 16.
  • Recent progress in generating intracellular functional antibody fragments to target and trace cellular components in living cells
    Kaiser PD, Maier J, Traenkle B, Emele F, Rothbauer U
    Biochim Biophys Acta. 2014 Nov;1844(11):1933-1942. doi: 10.1016/j.bbapap.2014.04.019. Epub 2014 May 2.
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