Dr. Anne Zeck

Areas of activity

Analytical chemistry with focus on chromatography and mass spectrometry of peptides and proteins, Detailed characterization of recombinant proteins and protein conjugates, Determination of identity / investigation of polyclonality, Determination of the coupling density of protein conjugates, High sensitivity sequence variant analysis, Determination of correct disulfide bridging, GlycosylationDetermination of N- and C-terminal modifications, Oxidation, deamidation, succinimide formation, glycation, etc., Epitope mapping using H/D exchange and top down mass spectrometry, Immunoanalytical methods for the production and characterization of antibodies

 

Stages of career

2007 - 2011 Group leader, Roche Diagnostics GmbH, Penzberg

2006 - 2007 Postdoc at Roche Diagnostics GmbH, Penzberg

2004 Postdoc at Barnett Institute of Chemical and Biological Analysis Northeastern University, Boston, USA

2001 Dr. rer. nat. in Analytical Chemistry, Technical University Munich (TUM)

1992 - 1998 Chemistry studies and Dipl. Chem. at the TU Karlsruhe and TUM

 

Dr. Anne Zeck
Group Leader Bioanalytics
Diplom-Chemikerin
T +49 (0)7121 51530-811

Publications

  • Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells
    Traenkle B, Kaiser PD, Pezzana S, Richardson J, Gramlich M, Wagner TR, Seyfried D, Weldle M, Holz S, Parfyonova Y, Nueske S, Scholz AM, Zeck A, Jakobi M, Schneiderhan-Marra N, Schaller M, Maurer A, Gouttefangeas C, Kneilling M, Pichler BJ, Sonanini D, Rothbauer U
    Front Immunol. 2021 Dec 9;12:799910. doi: 10.3389/fimmu.2021.799910. PMID: 34956237; PMCID: PMC8696186.
  • Altered Proinflammatory Responses to Polyelectrolyte Multilayer Coatings Are Associated with Differences in Protein Adsorption and Wettability
    Billing F, Walter B, Fink S, Arefaine E, Pickarski L, Maier S, Kretz R, Jakobi M, Feuerer N, Schneiderhan-Marra N, Burkhardt C, Templin M, Zeck A, Krastev R, Hartmann H, Shipp C.
    ACS Appl Mater Interfaces. 2021 Nov 24;13(46):55534-55549. doi: 10.1021/acsami.1c16175. Epub 2021 Nov 11. PMID: 34762399.
  • NeutrobodyPlex - monitoring SARS-CoV-2 neutralizing immune responses using nanobodies
    Wagner TR, Ostertag E, Kaiser PD, Gramlich M, Ruetalo N, Junker D, Haering J, Traenkle B, Becker M, Dulovic A, Schweizer H, Nueske S, Scholz A, Zeck A, Schenke-Layland K, Nelde A, Strengert M, Walz JS, Zocher G, Stehle T, Schindler M, Schneiderhan-Marra N, Rothbauer U
    EMBO Rep. 2021 May 5;22(5):e52325. doi: 10.15252/embr.202052325. Epub 2021 Apr 27. PMID: 33904225; PMCID: PMC8097376.
  • HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1
    Gramlich M, Hays HCW, Crichton S, Kaiser PD, Heine A, Schneiderhan-Marra N, Rothbauer U, Stoll D, Maier S, Zeck A
    Antibodies (Basel). 2021 Mar 19;10(1):11. doi: 10.3390/antib10010011. PMID: 33808657; PMCID: PMC8006148. Copy Downloa
  • Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity
    Becker M, Strengert M, Junker D, Kaiser P, Kerrinnes T, Traenkle B, Dinter H, Häring J, Ghozzi S, Zeck A, Weise F, Peter A, Hörber S, Fink S, Ruoff F, Dulovic A, Bakchoul T, Baillot A, Lohse S, Cornberg M, Illig T, Gottlieb J, Smola S, Karch A, Berger K, Rammensee HG, Schenke-Layland K, Nelde A, Märklin M, Heitmann JS, Walz JS, Templin M, Joos TO, Rothbauer U, Krause K & Schneiderhan-Marra N
    NATURE COMMUNICATIONS | (2021) 12:1152 | https://doi.org/10.1038/s41467-021-20973-3 | www.nature.com/naturecommunications
  • Cysteine in cell culture media induces acidic IgG1 species by disrupting the disulfide bond network
    Prade E, Zeck A, Stiefel F, Unsoeld A, Mentrup D, Arango Gutierrez E, Gorr IH
    Biotechnol Bioeng. 2021 Mar;118(3):1091-1104. doi: 10.1002/bit.27628. Epub 2020 Dec 16. PMID: 33200817; PMCID: PMC7986432.
  • NeutrobodyPlex - Nanobodies to monitor a SARS-CoV-2 neutralizing immune response
    Wagner T., Ostertag E., Kaiser P., Gramlich M., Ruetalo N., Junker D., Haering J., Traenkle B., Becker M., Dulovic A., Schweizer H., Nueske S., Scholz A., Zeck A., Schenke-Layland K., Nelde A., Strengert M., Walz J., Zocher G., Stehle T., Schindler M., Schneiderhan-Marra N., Rothbauer U
    In EMBO Reports 2021; 22(5):e52325. https://doi.org/10.15252/embr.202052325
  • Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes
    Guo RR, Comamala G, Yang HH, Gramlich M, Du YM, Wang T, Zeck A, Rand KD, Liu L, Voglmeir J
    Front Bioeng Biotechnol. 2020 Jul 3;8:741. doi: 10.3389/fbioe.2020.00741. PMID: 32719787; PMCID: PMC7348039.
  • Impact of S-sulfocysteine on fragments and trisulfide bond linkages in monoclonal antibodies
    Seibel R, Maier S, Schnellbaecher A, Bohl S, Wehsling M, Zeck A, Zimmer A
    MAbs. 2017 Aug/Sep;9(6):889-897. doi: 10.1080/19420862.2017.1333212. Epub 2017 Jun 5.
  • Pharmacodynamics of Oxaliplatin-Derived Platinum Compounds During Hyperthermic Intraperitoneal Chemotherapy (HIPEC): An Emerging Aspect Supporting the Rational Design of Treatment Protocols
    Loffler MW, Schuster H, Zeck A, Quilitz N, Weinreich J, Tolios A, Haen SP, Horvath P, Lob S, Rammensee HG, Konigsrainer I, Konigsrainer A, Beckert S
    Ann Surg Oncol. 2017 Jun;24(6):1650-1657. doi: 10.1245/s10434-017-5790-x. Epub 2017 Feb 3.
  • MASP-1 and MASP-2 Do Not Activate Pro-Factor D in Resting Human Blood, whereas MASP-3 Is a Potential Activator: Kinetic Analysis Involving Specific MASP-1 and MASP-2 Inhibitors
    Oroszlan G, Kortvely E, Szakacs D, Kocsis A, Dammeier S, Zeck A, Ueffing M, Zavodszky P, Pal G, Gal P, Dobo J
    J Immunol. 2016 Jan 15;196(2):857-65. doi: 10.4049/jimmunol.1501717. Epub 2015 Dec 16.
  • Determination of antibody glycosylation by mass spectrometry
    Jager C, Ferrara C, Umana P, Zeck A, Regula JT, Koll H
    Methods Mol Biol. 2012;901:195-208. doi: 10.1007/978-1-61779-931-0_13.
  • Low level sequence variant analysis of recombinant proteins: an optimized approach
    Zeck A, Regula JT, Larraillet V, Mautz B, Popp O, Gopfert U, Wiegeshoff F, Vollertsen UE, Gorr IH, Koll H, Papadimitriou A
    PLoS One. 2012;7(7):e40328. Epub 2012 Jul 6.
  • Cell Type-Specific and Site Directed N-Glycosylation Pattern of FcgammaRIIIa
    Zeck A, Pohlentz G, Schlothauer T, Peter-Katalinic J, Regula JT
    J. Proteome Res. 2011;10(7):3031–3039. DOI: 10.1021/pr1012653
  • Tris(hydroxymethyl)aminomethane Compatibility with N-Hydroxysuccinimide Ester Chemistry: Biotinylation of Peptides and Proteins in TRIS Buffer
    Kratzer U, Sommersdorf C, Maier S, Wagner TR, Templin M, Joos TO, Rothbauer U, Zeck A, Poetz O
    Bioconjug Chem. 2021 Sep 15;32(9):1960-1965. doi: 10.1021/acs.bioconjchem.1c00283. Epub 2021 Aug 18. PMID: 34406760.