Areas of activity
Analytical chemistry with focus on chromatography and mass spectrometry of peptides and proteins, Detailed characterization of recombinant proteins and protein conjugates, Determination of identity / investigation of polyclonality, Determination of the coupling density of protein conjugates, High sensitivity sequence variant analysis, Determination of correct disulfide bridging, GlycosylationDetermination of N- and C-terminal modifications, Oxidation, deamidation, succinimide formation, glycation, etc., Epitope mapping using H/D exchange and top down mass spectrometry, Immunoanalytical methods for the production and characterization of antibodies
Stages of career
2007 - 2011 Group leader, Roche Diagnostics GmbH, Penzberg
2006 - 2007 Postdoc at Roche Diagnostics GmbH, Penzberg
2004 Postdoc at Barnett Institute of Chemical and Biological Analysis Northeastern University, Boston, USA
2001 Dr. rer. nat. in Analytical Chemistry, Technical University Munich (TUM)
1992 - 1998 Chemistry studies and Dipl. Chem. at the TU Karlsruhe and TUM
Publications
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Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ CellsTraenkle B, Kaiser PD, Pezzana S, Richardson J, Gramlich M, Wagner TR, Seyfried D, Weldle M, Holz S, Parfyonova Y, Nueske S, Scholz AM, Zeck A, Jakobi M, Schneiderhan-Marra N, Schaller M, Maurer A, Gouttefangeas C, Kneilling M, Pichler BJ, Sonanini D, Rothbauer UFront Immunol. 2021 Dec 9;12:799910. doi: 10.3389/fimmu.2021.799910. PMID: 34956237; PMCID: PMC8696186.
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Altered Proinflammatory Responses to Polyelectrolyte Multilayer Coatings Are Associated with Differences in Protein Adsorption and WettabilityBilling F, Walter B, Fink S, Arefaine E, Pickarski L, Maier S, Kretz R, Jakobi M, Feuerer N, Schneiderhan-Marra N, Burkhardt C, Templin M, Zeck A, Krastev R, Hartmann H, Shipp C.ACS Appl Mater Interfaces. 2021 Nov 24;13(46):55534-55549. doi: 10.1021/acsami.1c16175. Epub 2021 Nov 11. PMID: 34762399.
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Tris(hydroxymethyl)aminomethane Compatibility with N-Hydroxysuccinimide Ester Chemistry: Biotinylation of Peptides and Proteins in TRIS BufferKratzer U, Sommersdorf C, Maier S, Wagner TR, Templin M, Joos TO, Rothbauer U, Zeck A, Poetz OBioconjug Chem. 2021 Sep 15;32(9):1960-1965. doi: https://doi.org/10.1021/acs.bioconjchem.1c00283. Epub 2021 Aug 18. PMID: 34406760.
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NeutrobodyPlex - monitoring SARS-CoV-2 neutralizing immune responses using nanobodiesWagner TR, Ostertag E, Kaiser PD, Gramlich M, Ruetalo N, Junker D, Haering J, Traenkle B, Becker M, Dulovic A, Schweizer H, Nueske S, Scholz A, Zeck A, Schenke-Layland K, Nelde A, Strengert M, Walz JS, Zocher G, Stehle T, Schindler M, Schneiderhan-Marra N, Rothbauer UEMBO Rep. 2021 May 5;22(5):e52325. doi: 10.15252/embr.202052325. Epub 2021 Apr 27. PMID: 33904225; PMCID: PMC8097376.
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HDX-MS for Epitope Characterization of a Therapeutic ANTIBODY Candidate on the Calcium-Binding Protein Annexin-A1Gramlich M, Hays HCW, Crichton S, Kaiser PD, Heine A, Schneiderhan-Marra N, Rothbauer U, Stoll D, Maier S, Zeck AAntibodies (Basel). 2021 Mar 19;10(1):11. doi: 10.3390/antib10010011. PMID: 33808657; PMCID: PMC8006148. Copy Downloa
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Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivityBecker M, Strengert M, Junker D, Kaiser P, Kerrinnes T, Traenkle B, Dinter H, Häring J, Ghozzi S, Zeck A, Weise F, Peter A, Hörber S, Fink S, Ruoff F, Dulovic A, Bakchoul T, Baillot A, Lohse S, Cornberg M, Illig T, Gottlieb J, Smola S, Karch A, Berger K, Rammensee HG, Schenke-Layland K, Nelde A, Märklin M, Heitmann JS, Walz JS, Templin M, Joos TO, Rothbauer U, Krause K & Schneiderhan-Marra NNATURE COMMUNICATIONS | (2021) 12:1152 | https://doi.org/10.1038/s41467-021-20973-3 | www.nature.com/naturecommunications
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Cysteine in cell culture media induces acidic IgG1 species by disrupting the disulfide bond networkPrade E, Zeck A, Stiefel F, Unsoeld A, Mentrup D, Arango Gutierrez E, Gorr IHBiotechnol Bioeng. 2021 Mar;118(3):1091-1104. doi: 10.1002/bit.27628. Epub 2020 Dec 16. PMID: 33200817; PMCID: PMC7986432.
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NeutrobodyPlex - Nanobodies to monitor a SARS-CoV-2 neutralizing immune responseWagner T., Ostertag E., Kaiser P., Gramlich M., Ruetalo N., Junker D., Haering J., Traenkle B., Becker M., Dulovic A., Schweizer H., Nueske S., Scholz A., Zeck A., Schenke-Layland K., Nelde A., Strengert M., Walz J., Zocher G., Stehle T., Schindler M., Schneiderhan-Marra N., Rothbauer UIn EMBO Reports 2021; 22(5):e52325. https://doi.org/10.15252/embr.202052325
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Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial GenomesGuo RR, Comamala G, Yang HH, Gramlich M, Du YM, Wang T, Zeck A, Rand KD, Liu L, Voglmeir JFront Bioeng Biotechnol. 2020 Jul 3;8:741. doi: 10.3389/fbioe.2020.00741. PMID: 32719787; PMCID: PMC7348039.
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Impact of S-sulfocysteine on fragments and trisulfide bond linkages in monoclonal antibodiesSeibel R, Maier S, Schnellbaecher A, Bohl S, Wehsling M, Zeck A, Zimmer AMAbs. 2017 Aug/Sep;9(6):889-897. doi: 10.1080/19420862.2017.1333212. Epub 2017 Jun 5.
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Pharmacodynamics of Oxaliplatin-Derived Platinum Compounds During Hyperthermic Intraperitoneal Chemotherapy (HIPEC): An Emerging Aspect Supporting the Rational Design of Treatment ProtocolsLoffler MW, Schuster H, Zeck A, Quilitz N, Weinreich J, Tolios A, Haen SP, Horvath P, Lob S, Rammensee HG, Konigsrainer I, Konigsrainer A, Beckert SAnn Surg Oncol. 2017 Jun;24(6):1650-1657. doi: 10.1245/s10434-017-5790-x. Epub 2017 Feb 3.
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MASP-1 and MASP-2 Do Not Activate Pro-Factor D in Resting Human Blood, whereas MASP-3 Is a Potential Activator: Kinetic Analysis Involving Specific MASP-1 and MASP-2 InhibitorsOroszlan G, Kortvely E, Szakacs D, Kocsis A, Dammeier S, Zeck A, Ueffing M, Zavodszky P, Pal G, Gal P, Dobo JJ Immunol. 2016 Jan 15;196(2):857-65. doi: 10.4049/jimmunol.1501717. Epub 2015 Dec 16.
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Determination of antibody glycosylation by mass spectrometryJager C, Ferrara C, Umana P, Zeck A, Regula JT, Koll HMethods Mol Biol. 2012;901:195-208. doi: 10.1007/978-1-61779-931-0_13.
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Low level sequence variant analysis of recombinant proteins: an optimized approachZeck A, Regula JT, Larraillet V, Mautz B, Popp O, Gopfert U, Wiegeshoff F, Vollertsen UE, Gorr IH, Koll H, Papadimitriou APLoS One. 2012;7(7):e40328. Epub 2012 Jul 6.
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Cell Type-Specific and Site Directed N-Glycosylation Pattern of FcgammaRIIIaZeck A, Pohlentz G, Schlothauer T, Peter-Katalinic J, Regula JTJ. Proteome Res. 2011;10(7):3031–3039. DOI: 10.1021/pr1012653