People

Marius Gramlich
PhD Bioanalytics
T +49 (0)7121 51530-817
F +49 (0)7121 51530-62
Robin Kretz
PhD student Bioanalytics
T +49 (0)7121 51530-890
F +49 (0)7121 51530-62
Sandra Maier
Scientist Bioanalytics
T +49 (0)7121 51530-817
F +49 (0)7121 51530-62
Eugenia Salzmann
PhD student Bioanalytics
T +49 (0)7121 51530-890
F +49 (0)7121 51530-62
Larissa Walter
PhD student Bioanalytics
T +49 (0)7121 51530-410
F +49 (0)7121 51530-62
Dr. Anne Zeck
Group Leader Bioanalytics
T +49 (0)7121 51530-811
F +49 (0)7121 51530-62

Research interests

Analytical chemistry with focus on chromatography and mass spectrometry of peptides and proteins

  • Detailed characterization of recombinant proteins and protein conjugates
  • Determination of identity / investigation of polyclonality
  • Determination of the coupling density of protein conjugates
  • High sensitivity sequence variant analysis
  • Determination of correct disulfide bridging
  • GlycosylationDetermination of N- and C-terminal modifications
  • Oxidation, deamidation, succinimide formation, glycation, etc.
  • Epitope mapping using H/D exchange and top down mass spectrometry
  • Immunoanalytical methods for the production and characterization of antibodies

Analytical Techniques

  • mass spectrometry
  • LTQ Orbitrap & LTQ-FT-ICR (ThermoScientific)
  • ESI-ToF and ESI-Q-ToF (MAXIS, Bruker)
  • MALDI-ToF-ToF
  • chromatography
  • HPLC, (Ultimate 3000, Agilent 1100, nanoAcquity)
  • UPLC, RSLC (nanoAcquity, Ultimate 3000)
  • On-line enrichment
  • immunonalytics
  • ELISA, immunoprecipitation, immunaffinity enrichment
  • Immunoanalytical detection of chromatographic separations

other protein analytical techniques

  • ion scarf chromatography
  • SDS-PAGE, Western Blot

 

Curriculum vitae

2007 - 2011 Group leader, Roche Diagnostics GmbH, Penzberg

2006 - 2007 Postdoc at Roche Diagnostics GmbH, Penzberg

2004 Postdoc at Barnett Institute of Chemical and Biological Analysis Northeastern University, Boston, USA

2001 Dr. rer. nat. in Analytical Chemistry, Technical University Munich (TUM)

1992 - 1998 Chemistry studies and Dipl. Chem. at the TU Karlsruhe and TUM

 

Student Assistants

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