Dr. Markus Templin - NMI.de

Cell Type-Specific Anti-Adhesion Properties of Peritoneal Cell Treatment with Plasma-Activated Media (PAM)

Holl, M., Rasch, M., Becker, L., Keller, A., Schultze-Rhonhof, L., Ruoff, F., Templin, M., Keller, S., Neis, F., Keßler, F., Andress, J., Bachmann, C., Krämer, B., Schenke-Layland, K., Brucker, S., Marzi, J., Weiss, M.

10.3390/biomedicines10040927

Noninvasive Physical Plasma as Innovative and Tissue-Preserving Therapy for Women Positive for Cervical Intraepithelial Neoplasia

Marzi, J., Stope, M., Henes, M., Koch, A., Wenzel, T., Holl, M., Layland, S., Neis, F., Bösmüller, H., Ruoff, F., Templin, M., Krämer, B., Stäbler, A., Barz, J., Berrio, D., Enderle, M., Loskill, P., Brucker, S., Schenke-Layland, K., Weiss, M.

10.3390/cancers14081933

Off-target lipid metabolism disruption by the mouse constitutive androstane receptor ligand TCPOBOP in humanized mice

Skoda J, Dohnalova K, Chalupsky K, Stahl A, Templin M, Maixnernova J, Micuda S, Grontved L, Bräuning A, Pavek P

Volume 197, March 2022, 114905; https://doi.org/10.1016/j.bcp.2021.114905

Altered Proinflammatory Responses to Polyelectrolyte Multilayer Coatings Are Associated with Differences in Protein Adsorption and Wettability

Billing F, Walter B, Fink S, Arefaine E, Pickarski L, Maier S, Kretz R, Jakobi M, Feuerer N, Schneiderhan-Marra N, Burkhardt C, Templin M, Zeck A, Krastev R, Hartmann H, Shipp C.

ACS Appl Mater Interfaces. 2021 Nov 24;13(46):55534-55549. doi: 10.1021/acsami.1c16175. Epub 2021 Nov 11. PMID: 34762399.

Targeted Protein Profiling of In Vivo NIPP-Treated Tissues Using DigiWest Technology

Ruoff, F.; Henes, M.; Templin, M.; Enderle, M.; Bösmüller, H.; Wallwiener, D.; Brucker, S.Y.; Schenke-Layland, K.; Weiss, M.

Appl. Sci. 2021, 11, 11238. https://doi.org/10.3390/app112311238

Characterization of hepatic zonation in mice by mass-spectrometric and antibody-based proteomics approaches

Kling S, Lang B, Hammer HS, Naboulsi W, Sprenger H, Frenzel F, Pötz O, Schwarz M, Braeuning A, Templin MF

Biol Chem. 2021 Oct 4. doi: 10.1515/hsz-2021-0314. Epub ahead of print. PMID: 34599868.

Tris(hydroxymethyl)aminomethane Compatibility with N-Hydroxysuccinimide Ester Chemistry: Biotinylation of Peptides and Proteins in TRIS Buffer

Kratzer U, Sommersdorf C, Maier S, Wagner TR, Templin M, Joos TO, Rothbauer U, Zeck A, Poetz O

Bioconjug Chem. 2021 Sep 15;32(9):1960-1965. doi: https://doi.org/10.1021/acs.bioconjchem.1c00283. Epub 2021 Aug 18. PMID: 34406760.

Advancement of precision oncology by integration of highly sensitive protein profiling technologies and patient-derived cell models for functional efficacy testing

Pawlak, M., Templin, M., & Schmees, C.

Immuno-Oncology Insights, 2(4), 171–185. https://doi.org/10.18609/ioi.2021.013

Antibody Response against SARS-CoV-2 and Seasonal Coronaviruses in Nonhospitalized COVID-19 Patients

Ruetalo N, Businger R, Althaus K, Fink S, Ruoff F, Pogoda M, Iftner A, Ganzenmüller T, Hamprecht K, Flehmig B, Bakchoul T, Templin MF, Schindler M

mSphere. 2021 Feb 24;6(1):e01145-20. doi: 10.1128/mSphere.01145-20. PMID: 33627511; PMCID: PMC8544894.

Nidogen-1 Mitigates Ischemia and Promotes Tissue Survival and Regeneration

Zbinden A, Layland SL, Urbanczyk M, Carvajal Berrio DA, Marzi J, Zauner M, Hammerschmidt A, Brauchle EM, Sudrow K, Fink S, Templin M, Liebscher S, Klein G, Deb A, Duffy GP, Crooks GM, Eble JA, Mikkola HKA, Nsair A, Seifert M, Schenke-Layland K

Adv Sci (Weinh). 2020 Dec 21;8(4):2002500. doi: 10.1002/advs.202002500. PMID: 33643791; PMCID: PMC7887579.

Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3D cultures

Schumacher D, Andrieux G, Boehnke K, Keil M, Silvestri A, Silvestrov M, Keilholz U, Haybaeck J, Erdmann G, Sachse C, Templin M, Hoffmann J, Boerries M, Schafer R, Regenbrecht CRA

PLoS Genet 15(3): e1008076. https://doi.org/10.1371/journal.pgen.1008076

Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3D cultures

Schumacher D, Andrieux G, Boehnke K, Keil M, Silvestri A, Silvestrov M, Keilholz U, Haybaeck J, Erdmann G, Sachse C, Templin M, Hoffmann J, Boerries M, Schäfer R, Regenbrecht C

PLoS Genet 15(3): e1008076.

Mouse Hepatomas with Ha-ras and B-raf Mutations Differ in Mitogen-Activated Protein Kinase Signaling and Response to Constitutive Androstane Receptor Activation

Braeuning A, Kollotzek F, Zeller E, Knorpp T, Templin MF, Schwarz M

Drug Metab Dispos. 2018 Nov;46(11):1462-1465. doi: 10.1124/dmd.118.083014. Epub 2018 Aug 16.

A high-throughput pipeline for validation of antibodies

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F

Nat Methods. 2018 Nov;15(11):909-912. doi: 10.1038/s41592-018-0179-8. Epub 2018 Oct 30.

Peptide-Based Sandwich Immunoassay for the Quantification of the Membrane Transporter Multidrug Resistance Protein

Poetz O, Dieze T, Hammer H, Weiß F, Sommersdorf C, Templin MF, Esdar C, Zimmermann A, Stevanovic S, Bedke J, Stenzl A, Joos TO.

Anal Chem. 2018 May 1;90(9):5788-5794. doi: 10.1021/acs.analchem.8b00152. Epub 2018 Apr 12.

Epidermal Growth Factor Represses Constitutive Androstane Receptor Expression in Primary Human Hepatocytes and Favors Regulation by Pregnane X Receptor.

de Boussac H, Gondeau C, Briolotti P, Duret C, Treindl F, Römer M, Fabre JM, Herrero A, Ramos J, Maurel P, Templin M, Gerbal-Chaloin S, Daujat-Chavanieu M

Drug Metab Dispos. 2018 Mar;46(3):223-236. doi: 10.1124/dmd.117.078683. Epub 2017 Dec 21.

Structure-dependent induction of apoptosis by hepatotoxic pyrrolizidine alkaloids in the human hepatoma cell line HepaRG: Single versus repeated exposure

Waizenegger J, Braeuning A, Templin M, Lampen A, Hessel-Pras S

Food Chem Toxicol. 2018 Apr;114:215-226. doi: 10.1016/j.fct.2018.02.036. Epub 2018 Feb 16.

Platelet protein biomarker panel for ovarian cancer diagnosis

Lomnytska M, Pinto R, Becker S, Engström U, Gustafsson S, Björklund C, Templin M, Bergstrand J, Xu L, Widengren J, Epstein E, Franzén B, Auer G.

Biomark Res. 2018 Jan 12;6:2. doi: 10.1186/s40364-018-0118-y.

Impact of serum and dialysates obtained from chronic hemodialysis patients maintained on high cut-off membranes on inflammation profile in human THP-1 monocytes

Trojanowicz B, Ulrich C, Fiedler R, Storr M, Boehler T, Martus P, Pawlak M, Glomb MA, Henning C, Templin M, Werner K, Zickler D, Willy K, Schindler R, Girndt M

Hemodial Int. 2017 Jul;21(3):348-358. doi: 10.1111/hdi.12494. Epub 2016 Sep 26.

Miniaturized Multiplexed Protein Binding Assays

Templin MF, Poetz O, Schwenk JM, Stoll D, Joos TO

W.-L. Xing and J. Cheng. Frontiers in Biochip Technology. Boston, MA: Springer US; 2006. 61-87.

Medium Cut-Off (MCO) Membranes Reduce Inflammation in Chronic Dialysis Patients-A Randomized Controlled Clinical Trial

Zickler D, Schindler R, Willy K, Martus P, Pawlak M, Storr M, Hulko M, Boehler T, Glomb MA, Liehr K, Henning C, Templin M, Trojanowicz B, Ulrich C, Werner K, Fiedler R, Girndt M

PLoS One. 2017 Jan 13;12(1):e0169024. doi: 10.1371/journal.pone.0169024. eCollection 2017.

Coordinating Role of RXRalpha in Downregulating Hepatic Detoxification during Inflammation Revealed by Fuzzy-Logic Modeling

Keller R, Klein M, Thomas M, Drager A, Metzger U, Templin MF, Joos TO, Thasler WE, Zell A, Zanger UM

PLoS Comput Biol. 2016 Jan 4;12(1):e1004431. doi: 10.1371/journal.pcbi.1004431. eCollection 2016 Jan.

High cut-off dialysis in chronic haemodialysis patients

Girndt M, Fiedler R, Martus P, Pawlak M, Storr M, Bohler T, Glomb MA, Liehr K, Henning C, Templin M, Trojanowicz B, Ulrich C, Werner K, Zickler D, Schindler R

Eur J Clin Invest. 2015 Dec;45(12):1333-40. doi: 10.1111/eci.12559.

RPPApipe: a pipeline for the analysis of reverse-phase protein array data

Eichner J, Heubach Y, Ruff M, Kohlhof H, Strobl S, Mayer B, Pawlak M, Templin MF

Biosystems. 2014 Aug;122:19-24. doi: 10.1016/j.biosystems.2014.06.009. Epub 2014 Jun 18.

EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer

Schneck, H., Gierke, B., Uppenkamp, F., Behrens, B., Niederacher, D., Stoecklein, N. H., Templin, M. F., Pawlak, M., Fehm, T., Neubauer, H.

Proteomics, 9(13), 3535–3548. https://doi.org/10.1002/pmic.200800159

A bead-based western for high-throughput cellular signal transduction analyses

Treindl F, Ruprecht B, Beiter Y, Schultz S, Dottinger A, Staebler A, Joos TO, Kling S, Poetz O, Fehm T, Neubauer H, Kuster B, Templin MF

Nat Commun. 2016 Sep 23;7:12852. doi: 10.1038/ncomms12852.

EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer

Schneck H, Gierke B, Uppenkamp F, Behrens B, Niederacher D, Stoecklein NH, Templin MF, Pawlak M, Fehm T, Neubauer H

PLoS One. 2015 Dec 22;10(12):e0144535. doi: 10.1371/journal.pone.0144535. eCollection 2015.

Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry

Weiß F, Schnabel A, Planatscher H, van den Berg BHJ, Serschnitzki B, Nuessler AK, Thasler WE, Weiss TS, Reuss M, Stoll D, Templin MF, Joos TO, Marcus K, Poetz O

Sci Rep. 2015 Mar 4;5:8759. doi: 10.1038/srep08759.

Bead-Based Peptide Arrays for Profiling the Specificity of Modification State-Specific Antibodies

Filomena A, Beiter Y, Templin MF, Joos TO, Schneiderhan-Marra N, Poetz O

Methods Mol Biol. 2015;1348:251-65. doi: 10.1007/978-1-4939-2999-3_22.

Cross-platform toxicogenomics for the prediction of non-genotoxic hepatocarcinogenesis in rat

Romer M, Eichner J, Metzger U, Templin MF, Plummer S, Ellinger-Ziegelbauer H, Zell A

PLoS One. 2014 May 15;9(5):e97640. doi: 10.1371/journal.pone.0097640. eCollection 2014.

Ha-ras and β-Catenin oncoproteins orchestrate metabolic programs in mouse liver tumors

Unterberger EB, Eichner J, Wrzodek C, Lempiäinen H, Luisier R, Terranova R, Metzger U, Plummer S, Knorpp T, Braeuning A, Moggs J, Templin MF, Honndorf V, Piotto M, Zell A, Schwarz M

International Journal of Cancer. 2014 Oct 1; 135(7): 1574-1585. https://doi.org/10.1002/ijc.28798

Multiple Protein Analysis of Formalin-fixed and Paraffin-embedded Tissue Samples with Reverse phase Protein Arrays

Assadi M, Lamerz J, Jarutat T, Farfsing A, Paul H, Gierke B, Breitinger E, Templin MF, Essioux L, Arbogast S, Venturi M, Pawlak M, Langen H, Schindler T

Mol Cell Proteomics. 2013 Sep;12(9):2615-22. doi: 10.1074/mcp.M112.023051. Epub 2013 May 7.

Multiple protein analysis of FFPE tissue samples with reverse-phase protein arrays

Assadi M, Lamerz J, Jarutat T, Farfsing A, Paul H, Gierke B, Breitinger E, Templin MF, Essioux L, Arbogast S, Venturi M, Pawlak M, Langen H, Schindler T

Mol Cell Proteomics. 2013 Sep;12(9):2615-22. doi: 10.1074/mcp.M112.023051. Epub 2013 May 7.

From spots to beads-PTM-peptide bead arrays for the characterization of anti-histone antibodies

Heubach Y, Planatscher H, Sommersdorf C, Maisch D, Maier J, Joos TO, Templin MF, Poetz O

Proteomics. 2013 Mar;13(6):1010-5. doi: 10.1002/pmic.201200383.

Identification of Dlk1-Dio3 imprinted gene cluster noncoding RNAs as novel candidate biomarkers for liver tumor promotion

Lempiainen H, Couttet P, Bolognani F, Muller A, Dubost V, Luisier R, Del Rio Espinola A, Vitry V, Unterberger EB, Thomson JP, Treindl F, Metzger U, Wrzodek C, Hahne F, Zollinger T, Brasa S, Kalteis M, Marcellin M, Giudicelli F, Braeuning A, Morawiec L, Zamurovic N, Langle U, Scheer N, Schubeler D, Goodman J, Chibout SD, Marlowe J, Theil D, Heard DJ, Grenet O, Zell A, Templin MF, Meehan RR, Wolf RC, Elcombe CR, Schwarz M, Moulin P, Terranova R, Moggs JG

Toxicol Sci. 2013 Feb;131(2):375-86. doi: 10.1093/toxsci/kfs303. Epub 2012 Oct 22.

Combining ultracentrifugation and peptide termini group-specific immunoprecipitation for multiplex plasma protein analysis

Volk S, Schreiber TD, Eisen D, Wiese C, Planatscher H, Pynn CJ, Stoll D, Templin MF, Joos TO, Poetz O

Mol Cell Proteomics. 2012 Jul;11(7):O111.015438. Epub 2012 Apr 23.

A Comprehensive Small Interfering RNA Screen Identifies Signaling Pathways Required for Gephyrin Clustering

Wuchter J, Beuter S, Treindl F, Herrmann T, Zeck G, Templin MF, Volkmer H

The Journal of Neuroscience. 2012 Oct 17; 32(42):14821–14834

Minimum information about a protein affinity reagent (MIAPAR)

Bourbeillon J, Orchard S, Benhar I, Borrebaeck C, Templin M, de Daruvar A, Dubel S, Frank R, Gibson F, Gloriam D, Haslam N, Hiltker T, Humphrey-Smith I, Hust M, Juncker D, Koegl M, Konthur Z, Korn B, et al.

NatBiotechnol. 2010 Jul;28(7):650-3. doi: 10.1038/nbt0710-650.

Targeting peptide termini - a novel immunoaffinity approach to reduce complexity in mass spectrometric protein identification

Hoeppe S, Schreiber TD, Planatscher H, Zell A, Templin MF, Stoll D, Joos TO, Poetz O

Mol Cell Proteomics. 2011 Feb;10(2):M110.002857. doi: 10.1074/mcp.M110.002857. Epub 2010 Oct 20.

Optimal selection of epitopes for TXP-immunoaffinity mass spectrometry

Planatscher H, Supper J, Poetz O, Stoll D, Joos T, Templin MF, Zell A

Algorithms Mol Biol. 2010 Jun 25;5:28. doi: 10.1186/1748-7188-5-28

Sequential multiplex analyte capturing for phosphor-protein profiling

Poetz O, Henzler T, Hartmann M, Kazmaier C, Templin MF, Herget T, Joos TO

Mol Cell Proteomics. 2010 Nov; 9(11): 2474–2481. Published online 2010 Aug 3. doi: 10.1074/mcp.M110.002709

Protein microarrays for diagnostic assays.

Hartmann M, Roeraade J, Stoll D, Templin MF, Joos TO

Anal Bioanal Chem. 2009 Mar;393(5):1407-16. Epub 2008 Sep 20. doi: 10.1007/s00216-008-2379-z.

On display on a bug: a systematic approach to characterize antibodies

Knorpp T, Templin MF

Nat Methods. 2008 Dec;5(12):1003-4. DOI: 10.1038/nmeth1208-1003

Novel functional profiling approach combining reverse phase protein microarrays and human 3-D ex vivo tissue cultures: expression of apoptosis-related proteins in human colon cancer.

Pirnia F, Pawlak M, Thallinger GG, Gierke B, Templin MF, Kappeler A, Betticher DC, Gloor B, Borner MM

Proteomics. 2009 Jul;9(13):3535-48. doi: 10.1002/pmic.200800159.

Proteome wide screening using peptide affinity capture

Poetz O, Hoeppe S, Templin MF, Stoll D, Joos TO

Proteomics. 2009 Mar;9(6):1518-23. doi: 10.1002/pmic.200800842.

Tumor promotion in liver of mice with a conditional Cx26 knockout.

Marx-Stoelting P, Mahr J, Knorpp T, Schreiber S, Templin MF, Ott T, Buchmann A, Schwarz M

Toxicol Sci. 2008 Jun;103(2):260-7. Epub 2008 Feb 27. doi: 10.1093/toxsci/kfn043

Expanding assay dynamics: a combined competitive and direct assay system for the quantification of proteins in multiplexed immunoassays

Hartmann M, Schrenk M, Dottinger A, Nagel S, Roeraade J, Joos TO, Templin MF

Clin Chem. 2008 Jun;54(6):956-63. Epub 2008 Apr 24. doi: 10.1373/clinchem.2007.099812

Increasing robustness and sensitivity of protein microarrays through microagitation and automation.

Hartmann M, Toegl A, Kirchner R, Templin MF, Joos TO

Anal Chim Acta. 2006 Mar 30;564(1):66-73. Epub 2006 Feb 21. DOI: 10.1016/j.aca.2006.01.041

Protein Microarrays - Neue Systeme für die Proteomforschung

Joos TO, Pawlak M, Templin M, Stoll D

GIT Labor-Fachzeitschrift. 2006; 10, page 890-892.

Protein microarrays: applications and future challenges

Stoll D, Templin MF, Bachmann J, Joos TO

Curr Opin Drug Discov Devel. 2005 Mar;8(2):239-52.

Protein Microarrays - A Promising Tool for Cancer Diagnosis

Schneiderhan-Marra N, Kirn A, Döttinger A, Templin M, Sauer G, Deissler H, Joos TO

Cancer Genomics & Proteomics. 2005 Jan; 2(1): 37-42.

Protein microarrays: catching the proteome.

Poetz O, Schwenk JM, Kramer S, Stoll D, Templin MF, Joos TO

Mech Ageing Dev. 2005 Jan;126(1):161-70. DOI: 10.1016/j.mad.2004.09.030

Protein microarrays for antibody profiling: Specificity and affinity determination on a chip.

Poetz O, Ostendorp R, Brocks B, Schwenk JM, Stoll D, Joos TO, Templin MF

Proteomics. 2005 Jun;5(9):2402-11. DOI: 10.1002/pmic.200401299

Protein microarrays and multiplexed sandwich immunoassays: what beats the beads?

Templin MF, Stoll D, Bachmann J, Joos TO

Comb Chem High Throughput Screen. 2004 May;7(3):223-9.

Microarray technology: an increasing variety of screening tools for proteomic research.

Stoll D, Bachmann J, Templin MF, Joos TO

DDT:Targets. 2004 Dec 15; 9(24): 10-7

Protein microarrays: promising tools for proteomic research

Templin MF, Stoll D, Schwenk JM, Pötz O, Kramer S, Joos TO

Proteomics. 2003 Nov;3(11):2155-66.

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