The surface interactions of Meibomian gland secretion (MGS) with polar lipid (PL), Egg Sphingomyelin (SM) or Dipalmitoylphosphatidylcholine (DPPC), are studied in mixed pseudo-binary films formed at the air/water interface of Langmuir surface balance. The behavior of the mixed films during slow quasi-equilibrium compression and during fast dynamic compression-decompression is registered by measurements of surface pressure and surface potential, and by monitoring film morphology with Brewster Angle Microscopy (BAM). Quasi-equilibrium compression isotherms are used to calculate the excess Gibbs and Helmholtz energy of mixing between MGS and PLs and thus to evaluate the interactions between the lipid compounds at the interface. The effects of PLs on the mixed film's elastic moduli of area compressibility, morphology and capability to attain high surface pressures are also examined. PLs interact with MGS with different strength and in different manner: MGS-SM interaction is weak and might lead to interfacial disaggregation of the thick meibium domains when SM is in excess, while MGS-DPPC interaction is strong and results in the formation of thick lipid aggregates. Both PLs increase the mixed films reciprocal compressibility and capability to achieve higher surface pressures. The results demonstrate that in vitro studies of the surface interactions between MGS and PLs might be beneficial in the selection of PLs for artificial tear formulations and for examination on molecular scale of the possible role of PLs at the ocular surface.