Non-contact protein microarray fabrication using a procedure based on liquid bridge formation.

Non-contact protein microarray fabrication using a procedure based on liquid bridge formation.
Hartmann M, Sjödahl J, Stjernström M, Redeby J, Joos T, Roeraade J
Anal Bioanal Chem. 2009 Jan;393(2):591-8. Epub 2008 Nov 21. doi: 10.1007/s00216-008-2509-7

Contemporary microarrayers of contact or non-contact format used in protein microarray fabrication still suffer from a number of problems, e.g. generation of satellite spots, inhomogeneous spots, misplaced or even absent spots, and sample carryover. In this paper, a new concept of non-contact sample deposition that reduces such problems is introduced. To show the potential and robustness of this pressure-assisted deposition technique, different sample solutions known to cause severe problems or to be even impossible to print with conventional microarrayers were accurately printed. The samples included 200 mg mL(-1) human serum albumin, highly concentrated sticky cell adhesion proteins, pure high-salt cell-lysis buffer, pure DMSO, and a suspension of 5-microm polystyrene beads. Additionally, a water-immiscible liquid fluorocarbon, which was shown not to affect the functionality of the capture molecules, was employed as a lid to reduce evaporation during microarray printing. The fluorocarbon liquid lid was shown to circumvent hydrolysis of water-sensitive activated surfaces during long-term deposition procedures.