Marker Independent Microscopy

Non-destructive imaging techniques for biomaterials and biological samples

Using endogenous fluorophores and molecule-specific spectral signatures - comparable to a characteristic fingerprint - images and data can be collected without affecting the sample state and also enables time-resolved, dynamic measurements.

Our services

Establishing and performing non-destructive and marker-independent microscopic procedures on synthetic and biological samples, especially for biomedical issues, such as:

• In vitro and in vivo degradation of biodegradable (implant) materials
• Real-time monitoring of pharmacological effects in in vitro cell culture models
• Investigation of morphological and molecular changes, e.g. in collagens

Methods

• Raman Spectroscopy & Microscopy, SERS and CARS Spectroscopy
• Multiphoton-Microscopy (TPEF, SHG, THG)
• Fluorescence- und Phosphorescence-Liftime Microscopy (FLIM, PLIM)
• Confocal Flourescence Microscopy
• 3D Image Reconstruction
• Multivariate data analysis and machine learning
• Spectral biomolecule databases

Devices

• InVia Raman Spectrometer
• Carl Zeiss LSM880