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Markus Templin
Dr. Markus Templin
Diplom-Biologe
Assayentwicklung
Tel.: 07121 51530-828
Fax: 07121 51530-16

Forschungsinteressen/Arbeitsgebiete:
Zelluläre Signalwege, Tumorbiologie, Biomarker-Identifizierung und Validierung, Assayentwicklung, miniaturisierte Assaysysteme

Wissenschaftlicher Werdegang mit den wichtigsten Stationen:
2000- heute NMI, ab 2003 Gruppenleiter
1996-2000 Wissenschaftlicher Mitarbeiter, Max-Planck-Institut für Entwicklungsbiologie, Tübingen
1994-1995 Postdoktorand, ICMB, University of Edinburgh, Scotland, UK

Publikationen

Treindl, F., Ruprecht, B., Beiter, Y., Schultz, S., Dottinger, A., Staebler, A., Joos, T. O., Kling, S., Poetz, O., Fehm, T., Neubauer, H., Kuster, B., Templin, M. F. (2016).
A bead-based western for high-throughput cellular signal transduction analyses
Nat Commun. 2016 Sep 23;7:12852. doi: 10.1038/ncomms12852.

Filomena, A., Beiter, Y., Templin, M. F., Joos, T. O., Schneiderhan-Marra, N., Poetz, O. (2015).
Bead-Based Peptide Arrays for Profiling the Specificity of Modification State-Specific Antibodies
Methods Mol Biol. 2015;1348:251-65. doi: 10.1007/978-1-4939-2999-3_22.

Templin, M. F., Poetz, O., Schwenk, J. M., Stoll, D., Joos, T. O. (2006).
Miniaturized Multiplexed Protein Binding Assays
Frontiers in Biochip Technology. W.-L. Xing and J. Cheng. Boston, MA, Springer US: 61-87.

Keller, R., Klein, M., Thomas, M., Drager, A., Metzger, U., Templin, M. F., Joos, T. O., Thasler, W. E., Zell, A., Zanger, U. M. (2016).
Coordinating Role of RXRalpha in Downregulating Hepatic Detoxification during Inflammation Revealed by Fuzzy-Logic Modeling
PLoS Comput Biol. 2016 Jan 4;12(1):e1004431. doi: 10.1371/journal.pcbi.1004431. eCollection 2016 Jan.

Schneck, H., Gierke, B., Uppenkamp, F., Behrens, B., Niederacher, D., Stoecklein, N. H., Templin, M. F., Pawlak, M., Fehm, T., Neubauer, H. (2015).
EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer
PLoS One. 2015 Dec 22;10(12):e0144535. doi: 10.1371/journal.pone.0144535. eCollection 2015.

Girndt, M., Fiedler, R., Martus, P., Pawlak, M., Storr, M., Bohler, T., Glomb, M. A., Liehr, K., Henning, C., Templin, M., Trojanowicz, B., Ulrich, C., Werner, K., Zickler, D., Schindler, R. (2015).
High cut-off dialysis in chronic haemodialysis patients
Eur J Clin Invest. 2015 Dec;45(12):1333-40. doi: 10.1111/eci.12559.

Eichner, J., Heubach, Y., Ruff, M., Kohlhof, H., Strobl, S., Mayer, B., Pawlak, M., Templin, M. F. (2014).
RPPApipe: a pipeline for the analysis of reverse-phase protein array data
Biosystems. 2014 Aug;122:19-24. doi: 10.1016/j.biosystems.2014.06.009. Epub 2014 Jun 18.

Lempiainen, H., Couttet, P., Bolognani, F., Muller, A., Dubost, V., Luisier, R., Del Rio Espinola, A., Vitry, V., Unterberger, E. B., Thomson, J. P., Treindl, F., Metzger, U., Wrzodek, C., Hahne, F., Zollinger, T., Brasa, S., Kalteis, M., Marcellin, M., Giudicelli, F., Braeuning, A., Morawiec, L., Zamurovic, N., Langle, U., Scheer, N., Schubeler, D., Goodman, J., Chibout, S. D., Marlowe, J., Theil, D., Heard, D. J., Grenet, O., Zell, A., Templin, M. F., Meehan, R. R., Wolf, R. C., Elcombe, C. R., Schwarz, M., Moulin, P., Terranova, R., Moggs, J. G. (2013).
Identification of Dlk1-Dio3 imprinted gene cluster noncoding RNAs as novel candidate biomarkers for liver tumor promotion
Toxicol Sci. 2013 Feb;131(2):375-86. doi: 10.1093/toxsci/kfs303. Epub 2012 Oct 22.

Romer, M., Eichner, J., Metzger, U., Templin, M. F., Plummer, S., Ellinger-Ziegelbauer, H., Zell, A. (2014).
Cross-platform toxicogenomics for the prediction of non-genotoxic hepatocarcinogenesis in rat
PLoS One. 2014 May 15;9(5):e97640. doi: 10.1371/journal.pone.0097640. eCollection 2014.

Weiß, F., Schnabel, A., Planatscher, H., van den Berg, B. H. J., Serschnitzki, B., Nuessler, A. K., Thasler, W. E., Weiss, T. S., Reuss, M., Stoll, D., Templin, M. F., Joos, T. O., Marcus, K., Poetz, O. (2015).
Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry
Sci. Rep. 5.

Unterberger, E. B., Eichner, J., Wrzodek, C., Lempiäinen, H., Luisier, R., Terranova, R., Metzger, U., Plummer, S., Knorpp, T., Braeuning, A., Moggs, J., Templin, M. F., Honndorf, V., Piotto, M., Zell, A., Schwarz, M. (2014).
Ha-ras and β-Catenin oncoproteins orchestrate metabolic programs in mouse liver tumors
International Journal of Cancer: n/a-n/a.

Assadi, M., Lamerz, J., Jarutat, T., Farfsing, A., Paul, H., Gierke, B., Breitinger, E., Templin, M. F., Essioux, L., Arbogast, S., Venturi, M., Pawlak, M., Langen, H., Schindler, T. (2013).
Multiple Protein Analysis of Formalin-fixed and Paraffin-embedded Tissue Samples with Reverse phase Protein Arrays
Mol Cell Proteomics. 2013 Sep;12(9):2615-22. doi: 10.1074/mcp.M112.023051. Epub 2013 May 7.

Assadi, M., Lamerz, J., Jarutat, T., Farfsing, A., Paul, H., Gierke, B., Breitinger, E., Templin, M. F., Essioux, L., Arbogast, S., Venturi, M., Pawlak, M., Langen, H., Schindler, T. (2013).
Multiple protein analysis of FFPE tissue samples with reverse-phase protein arrays
Mol Cell Proteomics. 2013 May 7.

Heubach, Y., Planatscher, H., Sommersdorf, C., Maisch, D., Maier, J., Joos, T. O., Templin, M. F., Poetz, O. (2013).
From spots to beads-PTM-peptide bead arrays for the characterization of anti-histone antibodies
Proteomics. 2013 Mar;13(6):1010-5. doi: 10.1002/pmic.201200383.

Volk, S., Schreiber, T. D., Eisen, D., Wiese, C., Planatscher, H., Pynn, C. J., Stoll, D., Templin, M. F., Joos, T. O., Potz, O. (2012)
Combining ultracentrifugation and peptide termini group-specific immunoprecipitation for multiplex plasma protein analysis
Mol Cell Proteomics. 2012 Jul;11(7):O111.015438. Epub 2012 Apr 23.

Wuchter, J., Beuter, S., Treindl, F., Herrmann, T., Zeck, G., Templin, MF., Volkmer, H. (2012)
A Comprehensive Small Interfering RNA Screen Identifies Signaling Pathways Required for Gephyrin Clustering
The Journal of Neuroscience, October 17, 32(42):14821–14834

Planatscher, H., Supper, J., Poetz, O., Stoll, D., Joos, T., Templin, M. F., Zell, A. (2010)
Optimal selection of epitopes for TXP-immunoaffinity mass spectrometry
Algorithms Mol Biol. 2010 Jun 25;5:28.

Hoeppe, S., Schreiber, T. D., Planatscher, H., Zell, A., Templin, M. F., Stoll, D., Joos, T. O., Poetz, O. (2010)
Targeting peptide termini - a novel immunoaffinity approach to reduce complexity in mass spectrometric protein identification
Mol Cell Proteomics. 2010 Oct 20.

Poetz, O., Henzler, T., Hartmann, M., Kazmaier, C., Templin, M. F., Herget, T., Joos, T. O. (2010)
Sequential multiplex analyte capturing for phospho-protein profiling
Mol Cell Proteomics mcp.M110.002709

Poetz, O., Henzler, T., Hartmann, M., Kazmaier, C., Templin, M. F., Herget, T., Joos, T. O. (2010)
Sequential multiplex analyte capturing for phospho-protein profiling
Mol Cell Proteomics. 2010 Nov;9(11):2474-81. Epub 2010 Aug 3

Bourbeillon, J., Orchard, S., Benhar, I., Borrebaeck, C., Templin, M., de Daruvar, A., Dubel, S., Frank, R., Gibson, F., Gloriam, D., Haslam, N., Hiltker, T., Humphrey-Smith, I., Hust, M., Juncker, D., Koegl, M., Konthur, Z., Korn, B., et al. (2010)
Minimum information about a protein affinity reagent (MIAPAR)
NatBiotechnol. 2010 Jul;28(7):650-3.

Pirnia, F., Pawlak, M., Thallinger, G. G., Gierke, B., Templin, M. F., Kappeler, A., Betticher, D. C., Gloor, B., Borner, M. M. (2009)
Novel functional profiling approach combining reverse phase protein microarrays and human 3-D ex vivo tissue cultures: expression of apoptosis-related proteins in human colon cancer.
Proteomics. 2009 Jul;9(13):3535-48.

Hartmann, M., Roeraade, J., Stoll, D., Templin, M.F., Joos, T.O. (2009)
Protein microarrays for diagnostic assays.
Anal Bioanal Chem. 2009 Mar;393(5):1407-16. Epub 2008 Sep 20.

Poetz, O., Hoeppe, S., Templin, M. F., Stoll, D., Joos, T. O. (2009)
Proteome wide screening using peptide affinity capture
Proteomics. 2009 Mar;9(6):1518-23.

Hartmann, M., Roeraade, J., Stoll, D., Templin, M. F., Joos, T. O. (2009)
Protein microarrays for diagnostic assays
Anal Bioanal Chem. 2009 Mar;393(5):1407-16. Epub 2008 Sep 20.

Stoll, D., Templin, M. F., Bachmann, J., Joos, T. O. (2005)
Protein microarrays: applications and future challenges
Curr Opin Drug Discov Devel. 2005 Mar;8(2):239-52.

Knorpp, T., Templin, M. F. (2009)
On display on a bug: a systematic approach to characterize antibodies
Nat Methods. 2008 Dec;5(12):1003-4.

Marx-Stoelting, P., Mahr, J., Knorpp, T., Schreiber, S., Templin, M. F., Ott, T., Buchmann, A., Schwarz, M. (2008)
Tumor promotion in liver of mice with a conditional Cx26 knockout.
Toxicol Sci. 2008 Jun;103(2):260-7. Epub 2008 Feb 27.

Hartmann, M., Schrenk, M., Dottinger, A., Nagel, S., Roeraade, J., Joos, T. O., Templin, M. F. (2008)
Expanding assay dynamics: a combined competitive and direct assay system for the quantification of proteins in multiplexed immunoassays
Clin Chem. 2008 Jun;54(6):956-63. Epub 2008 Apr 24.

Marx-Stoelting, P., Mahr, J., Knorpp, T., Schreiber, S., Templin, M. F., Ott, T., Buchmann, A., Schwarz, M. (2008)
Tumor Promotion in Liver of Mica with a Conditional Cx26 Knockout.
Toxicol Sci. 2008 Jun;103(2):260-7. Epub 2008 Feb 27.

Stoll, D., Bachmann, J., Templin, M.F., Joos, T.O. (2004)
Microarray technology: an increasing variety of screening tools for proteomic research.
DDT:Targets 3 (1) P. 24-31

Schneiderhan-Marra, N., Kirn, A., Döttinger, A., Templin, M., Sauer, G., Deissler, H., Joos, T.O. (2005)
Protein Microarrays - A Promising Tool for Cancer Diagnosis
Cancer Genomics & Proteomics Vol. 2, Issue 1, p. 37-42

Templin, M. F., Stoll, D., Bachmann, J., Joos, T. O. (2004)
Protein microarrays and multiplexed sandwich immunoassays: what beats the beads?
Comb Chem High Throughput Screen. 2004 May;7(3):223-9.

Templin, M. F., Stoll, D., Schwenk, J. M., Pötz, O., Kramer, S., Joos, T. O. (2003)
Protein microarrays: promising tools for proteomic research
Proteomics. 2003 Nov;3(11):2155-66.

Poetz, O., Schwenk, J. M., Kramer, S., Stoll, D., Templin, M. F., Joos, T. O. (2005)
Protein microarrays: catching the proteome.
Mech Ageing Dev. 2005 Jan;126(1):161-70.

Poetz, O., Ostendorp, R., Brocks, B., Schwenk, J. M., Stoll, D., Joos, T. O., Templin, M. F. (2005)
Protein microarrays for antibody profiling: Specificity and affinity determination on a chip.
Proteomics. 2005 Jun;5(9):2402-11.

Joos, T.O., Pawlak, M., Templin, M., Stoll, D. (2006)
Protein Microarrays - Neue Systeme für die Proteomforschung
GIT Labor-Fachzeitschrift 10, page 890-892.

Hartmann, M., Toegl, A., Kirchner, R., Templin, M.F., Joos , T. O. (2006)
Increasing robustness and sensitivity of protein microarrays through microagitation and automation.
Anal Chim Acta. 2006 Mar 30;564(1):66-73. Epub 2006 Feb 21.

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