personalised shRNA libraries
At the NMI personalised shRNA libraries (ENTRACE-technology) representing the individual transcriptome of patients are available. These are used for target and biomarker identification as well as elucidation of drug resistance mechanisms.
ENTRACE shRNA libraries are generated from individual patient transcriptomes and connect high throughput functional screenings with personalised approaches to investigate patient-specific cellular signaling pathways in tumorigenesis.
An important application for personalised ENTRACE libraries are functional screenings in the context of synthetic lethality.
Enzymatically generated transcriptome-based (ENTRACE) shRNA-libraries:
Using several enzymatic steps (ENTRACE-technology), personalised shRNA-libraries are generated from tumour tissue. Such libraries are employed in functional screens for target- and biomarker identification for personalised tumour-therapy.
Using the ENTRACE technology the transcriptome of individual patients is converted into an shRNA library, including knockdown-constructs against splice variants, mutations and formerly unknown transcripts.